Abstract
We evaluated a blend of medium-chain fatty acids (MCFA), organic acids, and a polyphenol antioxidant on gut integrity. Eighty Ross Broilers were exposed to 20-22°C (control - normothermic) or to 35-39.5°C (heat stress) for eight hours a day for a period of 1 or 5 days. Birds were fed a standard diet, or a diet supplemented with the test blend. Thereafter, birds were euthanized, and intestinal sections were excised for morphological, morphometric and gene expression analyses. Blood samples were collected for glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GSH-Px) activity and trolox equivalent antioxidant capacity (TEAC) determination. Heart and liver tissues were used to quantify the expression of heat shock proteins 60 and 70 (HSP60 and HSP70, respectively) and inhibitor of kappa light chain gene enhancer in B cells alpha (IKBA). The jejunum was the most sensitive intestinal section, where heat stress modulated the expression of HSP70, of the inflammatory markers IKBA, interleukin 8 (IL-8), interferon gamma (IFNγ), and toll-like receptor 4 (TLR4). Moreover, expression of tight junctions (CLDN1, ZO1 and ZO2) and nutrient transporters (PEPT1 and EAAT3) was modulated especially in the jejunum. In conclusion, the...Continue Reading
References
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·J Marder, Z Arad
Feb 23, 1995·Biochimica Et Biophysica Acta·H YasutakeS Takase
Jan 1, 1993·Annual Review of Genetics·D A Parsell, S Lindquist
Sep 21, 2000·Transplantation Proceedings·J S QuaedackersE Heineman
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·R M. RiveroL Romero
Mar 29, 2001·The Journal of Biological Chemistry·C H HeJ Alam
May 9, 2001·Nucleic Acids Research·M W Pfaffl
Sep 14, 2001·Cytokine·K ZachrissonA Uribe
Dec 6, 2002·The Biochemical Journal·Stefania FilosaGiuseppe Martini
Nov 7, 2003·Poultry Science·G B HavensteinM A Qureshi
Sep 10, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Carles GarrigaMiquel Moretó
Jan 13, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Karol DokladnyThomas Y Ma
Jul 14, 2007·Poultry Science·E R GilbertE A Wong
Oct 22, 2008·Poultry Science·C R MottE A Wong
Feb 25, 2009·Nucleic Acids Research·J M RuijterA F M Moorman
Feb 18, 2010·Life Sciences·Makiko TakadaSumio Watanabe
Jun 23, 2010·BMC Developmental Biology·Carlos I Lorda-DiezJuan M Hurle
Sep 21, 2010·Poultry Science·K SahinA Hayirli
Nov 27, 2010·The Journal of Surgical Research·Quan DuZheng-Guo Wang
Mar 25, 2011·The Journal of Nutrition·Dulantha UlluwishewaNicole C Roy
Sep 22, 2011·Poultry Science·S L Madsen, E A Wong
Feb 4, 2012·Journal of Cell Science·Mathieu NivonCarole Kretz-Remy
Mar 9, 2012·Poultry Science·X H GuX L Wang
Mar 22, 2012·International Journal of Biometeorology·Worapol Aengwanich, Maitree Suttajit
Aug 8, 2013·PloS One·Ann OsselaereSiska Croubels
Jan 21, 2014·Current Topics in Developmental Biology·David Wei-Min Tan, Nick Barker
Aug 20, 2014·Molecules and Cells·Hyun-Ju KimYoung-Ran Yoon
Nov 21, 2014·Avian Pathology : Journal of the W.V.P.A·Regiane R SantosJohanna Fink-Gremmels
Dec 3, 2014·Metabolism: Clinical and Experimental·Sarah J CarlsonMark Puder
Mar 26, 2015·Poultry Science·S DongI Zoccarato
Sep 25, 2015·PloS One·Soheil VarastehJohanna Fink-Gremmels
Jan 1, 2013·Animals : an Open Access Journal From MDPI·Lucas J Lara, Marcos H Rostagno
Jun 30, 2016·Journal of Animal Science and Biotechnology·Abdollah AkbarianStefaan De Smet
Aug 27, 2016·Phytotherapy Research : PTR·Sara A BurtRegiane R Santos
Sep 23, 2016·Oxidative Medicine and Cellular Longevity·Juan ZhangYuepu Pu
Jun 30, 2018·Journal of Agricultural and Food Chemistry·Xiaofang HeFeng Gao
Citations
Jul 17, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Qiufen LiGuanhong Li
Oct 3, 2020·Toxins·Nicole ReisingerBertrand Grenier
Jan 26, 2021·Frontiers in Veterinary Science·Vishwajit S ChowdhuryMitsuhiro Furuse
Jan 13, 2021·Journal of Animal Science and Biotechnology·Akshat GoelYang-Ho Choi
Mar 7, 2021·Toxins·Regiane R Santos, Ellen van Eerden
May 1, 2021·Animals : an Open Access Journal From MDPI·Regiane R SantosRonette Gehring
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Yordan MartínezManuel Valdivié
Jul 29, 2021·Veterinary World·Khaleel Emad KhaleelKhaled Musa Mohammad Saleh
Aug 1, 2021·Planta medica·Urszula LatekMarta Mendel