Effects of a feed additive blend on broilers challenged with heat stress

Avian Pathology : Journal of the W.V.P.A
Regiane R SantosJohanna Fink-Gremmels

Abstract

We evaluated a blend of medium-chain fatty acids (MCFA), organic acids, and a polyphenol antioxidant on gut integrity. Eighty Ross Broilers were exposed to 20-22°C (control - normothermic) or to 35-39.5°C (heat stress) for eight hours a day for a period of 1 or 5 days. Birds were fed a standard diet, or a diet supplemented with the test blend. Thereafter, birds were euthanized, and intestinal sections were excised for morphological, morphometric and gene expression analyses. Blood samples were collected for glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GSH-Px) activity and trolox equivalent antioxidant capacity (TEAC) determination. Heart and liver tissues were used to quantify the expression of heat shock proteins 60 and 70 (HSP60 and HSP70, respectively) and inhibitor of kappa light chain gene enhancer in B cells alpha (IKBA). The jejunum was the most sensitive intestinal section, where heat stress modulated the expression of HSP70, of the inflammatory markers IKBA, interleukin 8 (IL-8), interferon gamma (IFNγ), and toll-like receptor 4 (TLR4). Moreover, expression of tight junctions (CLDN1, ZO1 and ZO2) and nutrient transporters (PEPT1 and EAAT3) was modulated especially in the jejunum. In conclusion, the...Continue Reading

References

Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·J Marder, Z Arad
Feb 23, 1995·Biochimica Et Biophysica Acta·H YasutakeS Takase
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·R M. RiveroL Romero
Sep 10, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Carles GarrigaMiquel Moretó
Jan 13, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Karol DokladnyThomas Y Ma
Feb 25, 2009·Nucleic Acids Research·J M RuijterA F M Moorman
Nov 27, 2010·The Journal of Surgical Research·Quan DuZheng-Guo Wang
Mar 25, 2011·The Journal of Nutrition·Dulantha UlluwishewaNicole C Roy
Jan 21, 2014·Current Topics in Developmental Biology·David Wei-Min Tan, Nick Barker
Nov 21, 2014·Avian Pathology : Journal of the W.V.P.A·Regiane R SantosJohanna Fink-Gremmels
Dec 3, 2014·Metabolism: Clinical and Experimental·Sarah J CarlsonMark Puder
Jan 1, 2013·Animals : an Open Access Journal From MDPI·Lucas J Lara, Marcos H Rostagno
Jun 30, 2016·Journal of Animal Science and Biotechnology·Abdollah AkbarianStefaan De Smet

❮ Previous
Next ❯

Citations

Jan 26, 2021·Frontiers in Veterinary Science·Vishwajit S ChowdhuryMitsuhiro Furuse
Jan 13, 2021·Journal of Animal Science and Biotechnology·Akshat GoelYang-Ho Choi
May 1, 2021·Animals : an Open Access Journal From MDPI·Regiane R SantosRonette Gehring

❮ Previous
Next ❯

Methods Mentioned

BETA
protein folding

Software Mentioned

geNorm
MyiQ System

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.