Effects of a galacto-oligosaccharide-rich diet on fecal microbiota and metabolite profiles in mice

Food & Function
W ChengJing Yuan

Abstract

Galacto-oligosaccharides (GOS) are prebiotics that positively affect the host's gut microbiota, which is important for the health of the host. Most previous studies focused on specific flora components (e.g. Bifidobacterium and Lactobacillus); very few have investigated the relationship between flora and metabolites. Here, we used 16S rRNA analysis and metabolomics to analyze the effect of GOS on microbiota and metabolites. Results show that the abundance of Ruminococcaceae and Oscillibacter decreased significantly in GOS-fed mice. Twenty-one metabolites, including oleic acid, arachidic acid, and behenic acid, decreased significantly in the GOS-fed mice. Fatty acid synthesis and blood triglyceride content significantly decreased in the GOS-fed mice compared with those in the control mice, suggesting that GOS may improve lipid metabolism in mice. Additionally, after three weeks of a GOS-rich diet, the mouse microbiota was significantly enriched in Alloprevotella, Bacteroides, and Parasutterella. The blood glucose level of the GOS-fed group was significantly higher than that of the control group, whereas the abundance of Coprococcus and Odoribacter (butyrate-producing bacteria) was significantly decreased. The metabolism of butyr...Continue Reading

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Citations

Feb 13, 2020·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Ailing LiuJiaming Qian
Oct 1, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Hanieh-Sadat EjtahedBagher Larijani
Jul 22, 2019·Journal of Food and Drug Analysis·Ishu Khangwal, Pratyoosh Shukla
Aug 9, 2019·International Journal of Obesity Supplements·Giovanna MuscogiuriUNKNOWN on behalf of the Obesity Programs of nutrition, Education, Research and Assessment (OPERA) group
Jul 7, 2020·Frontiers in Cellular and Infection Microbiology·Liyuan ZhouMingqun Deng
Apr 15, 2020·Nutrients·Michele Pier Luca GuarinoMichele Cicala
May 13, 2020·Immune Network·Yoko M AmbrosiniHyun Jung Kim
Jun 27, 2018·Current Rheumatology Reports·Chiara Bellocchi, Elizabeth R Volkmann
Feb 19, 2020·Current Pharmaceutical Design·Jing GaoTieJun Li
Oct 2, 2020·International Journal of Radiation Oncology, Biology, Physics·Laura E EwingIgor Koturbash
Mar 23, 2021·Journal of Agricultural and Food Chemistry·Lei WangJianfa Zhang
Mar 28, 2021·Ageing Research Reviews·Jessica Conway, Niharika A Duggal
Apr 4, 2021·Biomolecules·Amrit Pal KaurDinesh Kumar
May 28, 2019·Journal of Agricultural and Food Chemistry·Zheng ZhangXiangna Zhao

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Methods Mentioned

BETA
electrophoresis
PCR
PCA

Software Mentioned

OPLS
PICRUSt
R
UPARSE
FLASH
SIMCA
Statistical Analysis of Metagenomic Profiles ( STAMP )
Trimmomatic
QIIME
DA

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