Effects of a novel biflavonoid of Lonicera japonica flower buds on modulating apoptosis under different oxidative conditions in hepatoma cells

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Haoqiang WanXiaobin Zeng

Abstract

In our previous work, we purified a novel biflavonoid named Japoflavone D (JFD) from Lonicera japonica flower buds. Biflavonoids are chemical compounds characterized by their high levels of antioxidative activity. The present study aimed to investigate the function and molecular mechanism of JFD under different oxidative conditions in hepatoma cells. MTT assay and apoptosis assay were used to evaluate the cytotoxic effect of JFD. The activities of SOD and CAT were detected to evaluate the oxidative level. Oxidative stress was induced by H2O2 stimulation. The molecular mechanism of JFD was investigated by analyzing relative signaling pathway. JFD inhibited cell viability in all hepatoma cell lines we examined. Under quiescent conditions, JFD treatment of SMMC-7721 cells resulted in upregulation of AKT/mTOR signal pathway and ERK activities and downregulation of KEAP1/NRF2/ARE signaling axis, together with apoptosis. However, under oxidative stress, JFD played a quite different role. Treatment of JFD suppressed the activation of ERK and mTOR and activated the KEAP1/NRF2/ARE signaling axis, which is a predominant regulator of cytoprotective responses to oxidative stress, thereby lessening the damage caused by excess reactive oxyge...Continue Reading

Citations

Aug 19, 2020·Journal of Ethnobiology and Ethnomedicine·Yong Xiong, Chunlin Long
Aug 21, 2019·Organic & Biomolecular Chemistry·Kui LuPeng Yu
Mar 7, 2020·Acta Crystallographica. Section C, Structural Chemistry·Zhaoxia QuFeng Zhao
Feb 4, 2021·Natural Products and Bioprospecting·Jean-François GoossensChristian Bailly
Mar 6, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·José C J M D S Menezes, Marc F Diederich
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Sep 23, 2020·European Journal of Pharmacology·Yuandong ZhuWenbin Chen

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis