Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model

European Journal of Nutrition
Conall R StrainPhilip J Allsopp

Abstract

Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate. These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined. This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID. Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h). Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cell...Continue Reading

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Citations

Jun 5, 2019·Marine Drugs·Paul CherryCatherine Stanton
Feb 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aroa Lopez-SantamarinaAlberto Cepeda
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Diane Purcell-MeyerinkMaria Hayes
Jul 3, 2021·Marine Drugs·Emer ShannonMaria Hayes

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Methods Mentioned

BETA
size-exclusion dialysis
PCR
PCRs
electrophoresis
size-exclusion filtration
size-exclusion chromatography

Software Mentioned

ANKOM
BLAST
PyNAST
QIIME
Varian Star Chromatography Workstation

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