Effects of a Potential Probiotic Strain Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-Induced Inflammatory Response and Gene Expression in Coinfected Gastric Epithelial Cells.

Probiotics and Antimicrobial Proteins
Mahdieh YarmohammadiMohammad Reza Zali

Abstract

In the present study, we aimed to investigate the modulatory effects of a potential probiotic bacterium Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-induced inflammatory response and gene expression in human gastric adenocarcinoma (AGS) cell line. The gastric epithelial cells were coinfected with a collection of H. pylori clinical strains alone or in combination with L. gasseri at a multiplicity of infection (MOI) of 1:100 for each bacterium, and incubated for different time points of 3, 6, and 12 h. IL-8 secretion from coinfected AGS cells after incubation at each time point was measured by an enzyme-linked immunosorbent assay (ELISA). The mRNA expression of IL-8, Bcl-2, β-catenin, integrin α5, and integrin β1 genes was determined by quantitative RT-PCR amplification of total RNA extracted from coinfected epithelial cells. L. gasseri significantly (P < 0.05 and P < 0.01) decreased the production of IL-8 in AGS cells coinfected with H. pylori strains at 6 h post-infection. We also detected that L. gasseri significantly (P < 0.05) down-regulated the gene expression level of IL-8 in H. pylori-stimulated AGS cells after 6 and 12 h of coinfection. Similarly, L. gasseri caused a significant decrease (P < 0.05) in mRNA exp...Continue Reading

References

Feb 1, 2002·FEMS Immunology and Medical Microbiology·Takao MukaiHitoshi Ohori
May 22, 2003·The International Journal of Biochemistry & Cell Biology·Joo Weon LimKyung Hwan Kim
Jul 16, 2003·Journal of Gastroenterology and Hepatology·Atsushi UshiyamaYasuhiro Koga
Mar 2, 2005·Nature Reviews. Cancer·J Martin Brown, Laura D Attardi
Apr 14, 2006·Alimentary Pharmacology & Therapeutics·M GottelandS Cruchet
May 2, 2006·International Journal of Gastrointestinal Cancer·Mohamed El-ShahatWesam A Nasif
Mar 27, 2007·Annals of the New York Academy of Sciences·Soon Ok ChoHyeyoung Kim
Jun 10, 2008·Applied and Environmental Microbiology·M Andrea Azcarate-PerilTodd R Klaenhammer
Feb 26, 2009·Letters in Applied Microbiology·K A RyanP W O'Toole
Dec 24, 2009·Nature Reviews. Cancer·Jay S Desgrosellier, David A Cheresh
May 5, 2012·Gut Microbes·Luciana Rodrigues da CunhaTodd Klaenhammer
Jan 16, 2013·Therapeutic Advances in Gastroenterology·Peera Hemarajata, James Versalovic
Jun 19, 2013·Best Practice & Research. Clinical Gastroenterology·Lars Engstrand, Mathilda Lindberg
Jun 25, 2013·FEMS Microbiology Reviews·Ines YangSebastian Suerbaum
Dec 24, 2013·World Journal of Gastroenterology : WJG·Ko Eun LeeYoung Do Jung
May 24, 2014·World Journal of Microbiology & Biotechnology·Abbas YadegarMohammad Reza Zali
May 21, 2015·World Journal of Experimental Medicine·Miguel Angel Chiurillo
Mar 1, 2012·Probiotics and Antimicrobial Proteins·Tejinder Pal SinghSuman Kapila
Dec 7, 2016·Frontiers in Cellular and Infection Microbiology·Ying HuangNong-Hua Lu
Feb 17, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Nastaran FarziMohammad Reza Zali
Oct 3, 2018·Helicobacter·Alice BlosseAlain P Gobert
Jan 17, 2019·Journal of Clinical Medicine·Yi-Hsing ChenChih-Ho Lai
Mar 12, 2019·Frontiers in Oncology·Khalid O AlfaroukSalvador Harguindey
May 16, 2019·World Journal of Gastroenterology : WJG·Sho SuzukiTakuji Gotoda

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