Effects of a single hit from the alpha hemolysin produced by Escherichia coli on the morphology of sheep erythrocytes.

Infection and Immunity
S E JorgensenG K Wu

Abstract

Scanning electron micrographs of sheep erythrocytes showed that attachment of the alpha hemolysin produced by Escherichia coli resulted in the formation of spherocytes, with 10 to 20 small projections spaced relatively evenly over the surface of the erythrocyte membrane. This shape change was induced within 5 min after treatment. If the hemolysin concentration was reduced to a level which would lyse only a fraction of the total erythrocytes, the affected cells were easily identified against a background of normal, unaffected cells. Unlike sodium lauryl sulfate and other amphipathic agents which enter cell membranes and increase their flexibility, low concentrations of hemolysin did not provide protection against hypotonic hemolysis. These findings indicate that the surface projections were not the result of membrane expansion caused by incorporation of hemolysin into the outer portion of the lipid bilayer. The ability of a given amount of hemolysin to release a constant amount of hemoglobin in the presence of increasing concentrations of red cells confirmed that a single hit is sufficient for lysis. These results suggest that a single hemolysin molecule can bind to a sheep erythrocyte and trigger internal reactions which result...Continue Reading

References

Jun 1, 1977·The Journal of Cell Biology·W Birchmeier, S J Singer
Mar 1, 1978·Proceedings of the Society for Experimental Biology and Medicine·J W EatonO Rundquist
May 1, 1976·Journal of Medical Microbiology·S E JorgensenG K Wu
Jul 1, 1976·The Journal of Cell Biology·M P Sheetz, S J Singer
May 1, 1974·Journal of Medical Microbiology·R P Rennie, J P Arbuthnott
Jan 1, 1966·Journal of Bacteriology·I S Snyder, N A Koch
Jun 1, 1961·Journal of Biochemistry·M NAKAOH YOSHIKAWA
Jan 1, 1963·The Journal of Pathology and Bacteriology·H W SMITH
May 1, 1971·Infection and Immunity·E C Short, H J Kurtz

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Citations

Mar 1, 1995·The Journal of Experimental Zoology·M R KasschauF N Watson
Mar 20, 2014·Infection and Immunity·Peter Svenssen MunksgaardHelle A Praetorius
May 27, 2014·The Journal of Biological Chemistry·Marianne SkalsHelle A Praetorius
Jun 1, 1988·European Journal of Epidemiology·S BhakdiI B Holland
Aug 19, 2011·Pflügers Archiv : European journal of physiology·Marianne SkalsHelle A Praetorius
Nov 9, 2011·The Journal of Membrane Biology·Casper K LarsenHelle A Praetorius
Jan 13, 2001·The Journal of Biological Chemistry·A L CortajarenaH Ostolaza
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Kathrin M FelderLudwig E Hoelzle
May 11, 2017·The Journal of Membrane Biology·Carl Martin SöderströmHelle A Praetorius
Aug 25, 2015·Biochimica Et Biophysica Acta·Laura C Ristow, Rodney A Welch
May 13, 2021·American Journal of Physiology. Cell Physiology·Helle Praetorius
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·P StanleyC Hughes
Dec 1, 1984·Microbiological Reviews·S J CavalieriI S Snyder
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·S E JorgensenC F Louis
Apr 1, 1986·Experimental Parasitology·M R Kasschau, M H Dresden

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