Effects of a single sickling event on the mechanical fragility of sickle cell trait erythrocytes.

Hemoglobin
Tennille D PresleyD B Kim-Shapiro

Abstract

Hemolysis contributes to the pathology associated with sickle cell disease. However, the mechanism of hemolysis or relative contribution of sickling due to hemoglobin (Hb) polymerization vs. oxidative damage remains unknown. Earlier studies aimed at deciphering the relative importance of these two mechanisms have been complicated by the fact that sickle red cells (SS) have already been affected by multiple rounds of sickling and oxidative damage before they are collected. In our study, we examine the mechanical fragility of sickle cell trait cells, which do not sickle in vivo, but can be made to do so in vitro. Thus, our novel approach explores the effects of sickle Hb polymerization on cells that have never been sickled before. We find that the mechanical fragility of these cells increases dramatically after a single sickling event, suggesting that a substantial amount of hemolysis in vivo probably occurs in polymer-containing cells.

References

Feb 1, 1978·The American Journal of Medicine·P R McCurdy, A S Sherman
Jan 1, 1990·Advances in Protein Chemistry·W A Eaton, J Hofrichter
Jul 1, 1986·The Biochemical Journal·C Rice-EvansE Baysal
Jan 1, 1985·Annual Review of Biophysics and Biophysical Chemistry·C T Noguchi, A N Schechter
May 1, 1985·The Journal of Clinical Investigation·B H RankR P Hebbel
Sep 1, 1985·American Journal of Hematology·G P RodgersA N Schechter
Apr 1, 1974·Archives of Internal Medicine·T A Bensinger, P N Gillette
Jul 8, 1966·Science·M Murayama
Sep 1, 1980·The Journal of Clinical Investigation·N MohandasS B Shohet
Dec 1, 1982·The Journal of Clinical Investigation·R P HebbelM H Steinberg
Feb 11, 1983·JAMA : the Journal of the American Medical Association·K J HelzlsouerA D Rogol
Dec 1, 1982·American Journal of Hematology·M L GulleyE P Orringer
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J BallaG M Vercellotti
Jun 8, 2001·American Journal of Hematology·Z HuangD B Kim-Shapiro
Jun 9, 2001·Antioxidants & Redox Signaling·A I AlayashR E Cashon
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·M AslanB A Freeman
Jul 20, 2002·Blood·Viktória JeneyGyörgy Balla
Nov 12, 2002·Nature Medicine·Christopher D ReiterMark T Gladwin
Feb 13, 2003·Current Opinion in Hematology·Christopher D Reiter, Mark T Gladwin
Mar 11, 2003·American Journal of Respiratory and Critical Care Medicine·Claudia R MorrisElliott P Vichinsky
Apr 11, 2003·The New England Journal of Medicine·Alan N Schechter, Mark T Gladwin
Jan 1, 1955·The American Journal of Medicine·W H CROSBY
Oct 1, 1960·British Journal of Haematology·H SCHUBOTHE, F P FOK
Sep 26, 2003·Biophysical Journal·Zhi HuangDaniel B Kim-Shapiro
Nov 15, 2003·BMJ : British Medical Journal·Susan Claster, Elliott P Vichinsky
Feb 27, 2004·The New England Journal of Medicine·Mark T GladwinFrederick P Ognibene
Mar 27, 2004·Nature Reviews. Drug Discovery·Abdu I Alayash
Apr 7, 2005·JAMA : the Journal of the American Medical Association·Russell P RotherMark T Gladwin
Jun 30, 2005·Blood·Vikki G NolanMartin H Steinberg
Nov 19, 2005·The Journal of Clinical Investigation·Peter C MinneciSteven B Solomon
Jul 20, 2007·The British Journal of Radiology·B HedayatiU Patel
May 15, 2008·Blood·Anne C FreiNancy J Wandersee

❮ Previous
Next ❯

Citations

Mar 26, 2014·Transfusion·Michael TarasevJed B Gorlin
Mar 4, 2014·American Journal of Hematology·Eduard J van BeersGregory J Kato
Oct 25, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Michael TarasevSumita Chakraborty
Dec 8, 2016·Artificial Organs·Luke A ZieglerMarina V Kameneva
May 22, 2020·Nature Reviews. Materials·Yongzhi QiuWilbur A Lam
Jan 19, 2021·Clinical Hemorheology and Microcirculation·Jennell WhitePatrick C Hines

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.