PMID: 557989May 3, 1977Paper

Effects of a trinucleotide ethyl phosphotriester, Gmp(Et)Gmp(Et)U, on mammalian cells in culture

Biochemistry
P S MillerP O Ts'o

Abstract

The nonionic 2'-O-methyribooligonucleotide ethyl phosphotriester, Gmp(Et)Gmp(Et)U, is complementary to the...ApCpC...sequence found in the amino acid accepting stem of most tRNAs and the anticodon region of tRNAgly and to the threonine codon of mRNA. Gmp(Et)Gmp(EtU forms hydrogen-bonded complexes with the amino acid accepting stem of tRNApheyeast and unfractionated tRNA Escherichia coli under physiological salt conditions at 37 degrees C as determined by equilibrium dialysis. The extent of phenylalanine aminoacylation of tRNApheE.coli is inhibited 39% by Gmp(Et)Gmp(Et)U at 37 degrees C in solution. The triester is resistant to hydrolysis by serum nucleases and cell lysates. The triester is readily taken up by transformed Syrian hamster fibroblasts growing in monolayer. Within the cell, the triester is deethylated to give the trinucleotide species Gmp(Et)GmpU, GmpGmp(Et)U, and GmpGmpU and is also hydrolyzed to dimeric and monomeric units. Treatment of transformed fibroblasts in monolayer with 25 micronM Gmp(Et)Gmp(Et)U results in a 40% inhibition of cellular protein synthesis with a concurrent slight increase in cellular RNA synthesis during the first 4 h. After 4 h, the rate of cellular protein synthesis begins to recover while...Continue Reading

Associated Clinical Trials

Citations

Apr 9, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·A MurakamiN Wake
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P C Zamecnik, M L Stephenson
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K JayaramanP O Ts'o
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D R ElmalehE Rapaport
Jan 1, 1978·Preparative Biochemistry·R H Hayashikawa, J Nagyvary
Oct 10, 1979·Nucleic Acids Research·K K Ogilvie, S L Beaucage
Jan 1, 1990·Annals of the New York Academy of Sciences·G Zon
Mar 14, 2014·Expert Opinion on Biological Therapy·Aleksander TouznikToshifumi Yokota
Oct 28, 1992·Annals of the New York Academy of Sciences·P O Ts'oP S Miller
Mar 17, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·J R ThayerC A Pohl
Apr 21, 2009·Bioorganic & Medicinal Chemistry Letters·Koji NagahamaJesper Wengel
Jul 1, 1990·Analytical Biochemistry·V F Zarytova, I G Shishkina
May 1, 1990·Bioconjugate Chemistry·P S Miller
Jan 1, 2013·Journal of Personalized Medicine·Joshua J A Lee, Toshifumi Yokota
Aug 25, 2012·Organic & Biomolecular Chemistry·Atsuo ShigenoKenzo Fujimoto
Jun 21, 1990·Biochimica Et Biophysica Acta·C Hélène, J J Toulmé
Aug 31, 2021·RSC Chemical Biology·Guillaume ClavéMichael Smietana
Oct 23, 2004·Science·Steven A Benner

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