Effects of ACE inhibition on spontaneous and insulin-stimulated endothelin-1 secretion: in vitro and in vivo studies

Diabetes
G DesideriA Santucci

Abstract

To evaluate the effect of angiotensin-converting enzyme inhibition on spontaneous and insulin-stimulated endothelin-1 (ET-1) secretion in vitro and in vivo, human endothelial cells derived from umbilical cord veins were cultured onto acellular collagen-coated permeable membrane, thus mimicking in vivo conditions with a luminal and abluminal side. Insulin (10(-6,-8,-9) mol/l) significantly stimulated ET-1 secretion by cultured cells (P < 0.05 starting from 2-h incubation). Captopril (10(-7,-8,-9) mol/l) significantly reduced both spontaneous and insulin-stimulated ET-1 secretion, while increasing nitric oxide production. Considering each cell side, captopril significantly inhibited the apical secretion of ET-1, while its effect on the basolateral compartment was modest. In the presence of D-Arg,[Hyp3,Thi5,8,D-Phe7]-bradykinin (10(-6) mol/l), a bradykinin B2 receptor antagonist, captopril had no effects on ET-1 and nitric oxide production and also when insulin was added to the culture media. With regard to in vivo experiments, oral captopril therapy (25 mg twice daily for 1 week) was given to normotensive (n = 5) and hypertensive (n = 6) subjects and significantly decreased plasma ET-1 concentration (normotensive subjects, before...Continue Reading

Citations

May 3, 2002·Journal of the American College of Cardiology·Joerg HerrmannAmir Lerman
Sep 15, 1998·Kidney International. Supplement·M HermannG A Müller
Jun 19, 2003·The Journal of Clinical Investigation·Tatsuya KondoC Ronald Kahn
May 10, 2012·Molecular Endocrinology·Vincenza CifarelliH Henry Dong
Oct 13, 2000·European Journal of Clinical Investigation·C G SchalkwijkC D Stehouwer
Sep 9, 2008·Endocrinology and Metabolism Clinics of North America·Ranganath MuniyappaMichael J Quon
Jul 25, 2007·Journal of Internal Medicine·P-A Jansson
Jul 16, 2005·The American Journal of Cardiology·Jaume FiguerasJordi Soler Soler
May 9, 2002·The Annals of Thoracic Surgery·Lawrence KoSubodh Verma
May 26, 2007·Endocrine Reviews·Ranganath MuniyappaMichael J Quon
Nov 10, 2001·The Journal of Clinical Endocrinology and Metabolism·W BaggG D Braatvedt
Mar 20, 2003·Current Diabetes Reports·Dmitri KirpichnikovJames R Sowers
Jul 17, 2010·Mediators of Inflammation·Inge A M van den OeverSuat Simsek
Nov 9, 2000·Circulation·G DesideriC Ferri
Jul 30, 2008·Endothelium : Journal of Endothelial Cell Research·Vasileios F PanoulasGeorge D Kitas
May 24, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Giovambattista DesideriClaudio Ferri
Aug 31, 2000·Journal of the American Society of Nephrology : JASN·Kerstin AmannEberhard Ritz

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