PMID: 9438518Jan 23, 1998Paper

Effects of acetaldehyde on brush border enzyme activities in human colon adenocarcinoma cell line Caco-2

Alcoholism, Clinical and Experimental Research
T Koivisto, M P Salaspuro

Abstract

The treatment of Caco-2 cells, a human colon adenocarcinoma cell line that closely resembles normal human small intestinal epithelial cells, with acetaldehyde resulted in significantly decreased activities of brush border enzymes sucrase, maltase, lactase, and gamma-glutamyltransferase; alkaline phosphatase activity was not affected. In the case of sucrase and maltase, the activities were also decreased by a combination of acetaldehyde and ethanol, although ethanol alone markedly increased them. The possibility that intraintestinal acetaldehyde, formed by intestinal microbes, might play a role in some small intestinal enzyme deficiencies observed earlier in alcoholics should therefore be considered. The mechanism by which acetaldehyde alters these enzyme activities remains unclear. The observation that acetaldehyde also disturbed cell polarization, an initial step in the process of differentiation in Caco-2 cells, indicates that acetaldehyde might decrease these enzyme activities by interfering with cell differentiation. Because ethanol and acetaldehyde metabolizing enzymes have not been previously studied from Caco-2 cells, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activities were also measured from these c...Continue Reading

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Citations

Jun 28, 2003·Best Practice & Research. Clinical Gastroenterology·Mikko P Salaspuro
Nov 30, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Simonetta FerruzzaYula Sambuy
May 29, 2004·Alcoholism, Clinical and Experimental Research·P ShethR K Rao
Feb 1, 2005·European Journal of Pharmacology·Youn Ju Lee, Shivendra D Shukla
Dec 3, 2014·Redox Biology·Christopher B ForsythAli Keshavarzian
Jul 1, 2014·Food & Function·A HeinleinA Buettner
Dec 3, 1998·Alcoholism, Clinical and Experimental Research·R K Rao
Feb 19, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·S BasuroyR K Rao
May 11, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Christopher B ForsythAli Keshavarzian
Oct 8, 2004·The Journal of Pharmacology and Experimental Therapeutics·Jan S MorebDagmara Mohuczy
Oct 13, 2009·The Journal of Pharmacology and Experimental Therapeutics·Scott J FisherNatalie D Eddington

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