PMID: 4291730Jan 1, 1967Paper

Effects of acetylcholine and other medullary secretagogues and antagonists on the membrane potential of adrenal chromaffin cells: an analysis employing techniques of tissue culture

The Journal of Physiology
W W DouglasS R Sampson

Abstract

1. A method has been devised for isolating adrenal chromaffin cells (from gerbils) and maintaining them in vitro. Transmembrane potentials of these cells were recorded with intracellular micro-electrodes.2. Acetylcholine depolarized the chromaffin cells and so did various other substances known to evoke catecholamine secretion: nicotine, pilocarpine, histamine, 5-hydroxytryptamine, angiotensin, and bradykinin.3. The depolarizing effect of acetylcholine was partially antagonized by hexamethonium and was blocked completely by hexamethonium in combination with atropine.4. Hexamethonium alone completely blocked the response to nicotine; and atropine alone abolished the response to pilocarpine. Thus both nicotinic and muscarinic receptors are present in gerbil chromaffin cells.5. The experiments demonstrate that the various secretagogues and antagonists tested act on the plasma membrane of the chromaffin cell and raise the question whether depolarization may be an important event in stimulus-secretion coupling.

Citations

Feb 15, 1974·Experientia·O H Petersen
Dec 21, 1973·Zeitschrift für Zellforschung und mikroskopische Anatomie·K Unsicker
Dec 31, 1973·Zeitschrift für Zellforschung und mikroskopische Anatomie·K Unsicker
Jan 19, 2012·Journal of Molecular Neuroscience : MN·Nathalie C GuérineauEmilio Carbone
Jan 1, 1983·Journal of the Autonomic Nervous System·B G LivettM H Lindenbaum
Sep 24, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·M GöthertI Loewenstein
Jan 1, 1975·Naunyn-Schmiedeberg's Archives of Pharmacology·H KilbingerE Muscholl
Apr 2, 2003·Pharmacology & Therapeutics·Philip D Marley
Dec 22, 1999·British Journal of Pharmacology·P J BalesP D Marley
Dec 4, 1989·Neuroscience Letters·L L MinerB B Kaplan
Sep 1, 1985·Archives internationales de physiologie et de biochimie·A C GavottoS B Villagra
Nov 1, 1977·Clinics in Endocrinology and Metabolism·R L Perlman, M Chalfie
Jan 1, 1970·Progress in Biophysics and Molecular Biology·J I Hubbard
Jul 23, 2003·British Journal of Pharmacology·Silvia Di AngelantonioAndrea Nistri
Nov 21, 2007·Acta Physiologica·L Olivos, A R Artalejo
Nov 1, 1982·British Journal of Pharmacology·S M KirpekarM T Schiavone
Jul 29, 1985·Brain Research·O HäppöläP Panula
Mar 1, 1975·British Journal of Pharmacology·R P Rubin, W Warner
Apr 1, 1977·Journal of Neurochemistry·S P Wilson, N Kirshner
Feb 1, 1968·Journal of Cellular Physiology·M R Swift, G J Todaro
Jul 1, 1974·Acta diabetologica latina·P Baráth
Feb 1, 1982·Journal of Neurochemistry·D L KilpatrickN Kirshner
Jul 1, 1982·Journal of Neurochemistry·C Amy, N Kirshner
Sep 1, 1980·Journal of Neurochemistry·D L KilpatrickN Kirshner
Jan 1, 1977·Journal of Neurology, Neurosurgery, and Psychiatry·M Takamori
Oct 3, 1998·The Japanese Journal of Physiology·T Kanno
Apr 1, 1986·European Journal of Biochemistry·S KotaniH Sakai
Sep 22, 2007·Journal of Neurochemistry·Alberto Pérez-Alvarez, Almudena Albillos
Nov 1, 1973·Biulleten' eksperimental'noĭ biologii i meditsiny·I S GushchinN L Tsziu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.