Effects of acetylcholine on lung liquid production by in vitro lungs from fetal guinea pigs

Canadian Journal of Physiology and Pharmacology
B A WoodsA M Perks

Abstract

Lungs from near-term fetal guinea pigs (61 +/- 2 days of gestation) were supported in vitro for 3 h; lung liquid production was monitored by a dye dilution method. Untreated preparations produced lung liquid with no significant changes (ANOVA; regression analysis) (rates in successive hours, initial study: 1.37 +/- 0.30, 1.36 +/- 0.30, and 1.28 +/- 0.27 mL.kg-1 body weight.h-1; n = 6). Preparations given acetylcholine at 10(-4) (n = 6), 10(-5) (n = 6), and 10(-6) M (n = 18) during the middle hour showed marked and significant fluid reabsorption (p < 0.025-0.0005); 10(-8) M acetylcholine was without effect. Reductions were linearly related to log concentration of acetylcholine (r = 0.97; theoretical threshold, 1.0 x 10(-7) M acetylcholine). Atropine, at 10(-5) M, greatly reduced responses to acetylcholine, and all reabsorptions were abolished; 10(-4) M atropine completely abolished all responses to acetylcholine; atropine alone had no effect (based on 48 studies). The alpha-adrenoreceptor antagonist phentolamine (1.78 x 10(-5) M) abolished the effects of 10(-6) M acetylcholine, but had no effect alone (based on 48 studies); the beta-adrenoreceptor antagonist propranolol (10(-5) M) had no effect on responses to 10(-6) M acetylcho...Continue Reading

References

Mar 1, 1978·Pediatric Research·D V Walters, R E Olver
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Jan 1, 1988·The Journal of Physiology·D Colquhoun, D C Ogden
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Citations

Jun 29, 2000·Archives of Disease in Childhood. Fetal and Neonatal Edition·N ChooA M Perks
Oct 23, 1998·The Journal of Physiology·B A Chua, A M Perks
Jan 8, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yoshizumi TakemuraDouglas C Eaton

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