Effects of acoustic streaming from moderate-intensity pulsed ultrasound for enhancing biofilm mitigation effectiveness of drug-loaded liposomes

The Journal of the Acoustical Society of America
Dong MaJunru Wu

Abstract

Because biofilms have resistance to antibiotics, their control using minimum amounts of chemicals and energy becomes a critical issue particularly for resource-constrained long-term space and deep-sea explorations. This preliminary study investigates how ultrasound promoting penetration of antibiotic-loaded liposomes into alginate-based bacterial biofilms, resulting in enhanced bacterial (Ralstonia insidiosa) killing. Nano-sized liposomes are used as a delivery vehicle for the antibiotic gentamicin. Alginate-based synthetic biofilms, which are widely acknowledged as biofilm phantoms, filled with liposome solution are formed at the bottoms of six-well Petri dishes and exposed to ultrasound (frequency = 2.25 MHz, 10% duty cycle, and spatially and temporally averaged intensity ISAPA = 4.4 W/cm(2)). Gentamicin is released from liposomes after they are lysed using detergent solution (0.05% sodium dodecyl sulfate, 1.0% Triton X-100) and incubated for 20 min. The alginate biofilm is dissolved and diluted, counting of colony-forming units shows about 80% of the bacteria are killed. It has also been shown the liposome-capture density by the alginate film increases linearly with the ultrasound intensity up to ISAPA = 6.2 W/cm(2) reaching...Continue Reading

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Citations

Jul 3, 2016·The Journal of the Acoustical Society of America·Jeffrey S Marshall
May 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roberto CanaparoLoredana Serpe
Jan 4, 2017·The Journal of the Acoustical Society of America·Dong Ma, Junru Wu
Jan 3, 2019·The Journal of the Acoustical Society of America·Dong MaJunru Wu
Jan 5, 2021·Critical Reviews in Food Science and Nutrition·Dan LiuXin-An Zeng

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