Effects of acrylamide in the presence of vitamin E on sperm parameters, chromatin quality, and testosterone levels in mice

Clinical and Experimental Reproductive Medicine
M AnvariM Pourentezari

Abstract

The present study investigated sperm chromatin quality and testosterone levels in acrylamide-treated mice and the possible protective effects of vitamin E on the fertility potential of spermatozoa. Thirty-two adult male mice were divided equally into four groups. Group 1 was the control, group 2 received acrylamide (10 mg/kg, water solution), group 3 received vitamin E (100 mg/kg, intraperitoneal), and group 4 received both acrylamide and vitamin E. After 35 days, spermatozoa from the right cauda epididymis were analyzed in terms of count, motility, morphology, and viability. Sperm DNA integrity and chromatin condensation were assessed by acridine orange (AO), aniline blue (AB), toluidine blue (TB), and chromomycin A3 (CMA3) staining. In acrylamide-treated mice, significantly lower sperm concentration, viability, motility, and testosterone levels were found in comparison with the control and acrylamide+vitamin E groups (p<0.05). In the vitamin E group, significantly more favorable sperm parameters and testosterone levels were found than in the other groups (p<0.05). There were also significantly more spermatozoa with less condensed chromatin in the acrylamide-treated mice than in the other groups. Moreover, significantly more s...Continue Reading

References

Dec 1, 1996·Urology·R K Sharma, A Agarwal
Mar 18, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Luchuan CaoSalman Azhar
Mar 8, 2005·Reproductive Toxicology·Hye-Jin YangChang-Hoon Han
Dec 6, 2011·Asian Journal of Andrology·Lara TamburrinoMonica Muratori
Feb 2, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Suna KalenderAyse Aslanturk
Jan 30, 2014·Nutrients·Joanna Fiedor, Květoslava Burda
Aug 16, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Ali Reza TalebiIman Halvaei
Jun 20, 2015·Human Fertility : Journal of the British Fertility Society·Josep Gual-FrauJuan Prats
Jun 28, 2016·Cell and Tissue Banking·Fardin AmidiSaeid Nekoonam
Mar 27, 2018·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jianxia SunWeibin Bai
Aug 28, 2018·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Demetris KafourisStelios Yiannopoulos

❮ Previous
Next ❯

Citations

Jun 6, 2021·Molecular & Cellular Proteomics : MCP·David A Skerrett-ByrneJohn E Schjenken
Jan 16, 2022·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hang ZhangTao Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
light microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Iranian Journal of Reproductive Medicine
Mohammad MardaniShahnaz Razavi
European Journal of Obstetrics, Gynecology, and Reproductive Biology
Mohammad Kermani-AlghoraishiSeyed Mohsen Miresmaili
© 2022 Meta ULC. All rights reserved