Effects of ACTH and cAMP on steroidogenic acute regulatory protein and P450 11beta-hydroxylase messenger RNAs in rainbow trout interrenal cells: relationship with in vitro cortisol production

General and Comparative Endocrinology
I Julie HagenGraham Young

Abstract

Steroidogenic acute regulatory protein (StAR) transfers cholesterol over the inner mitochondrial membrane, thereby making the molecule available for cholesterol side-chain cleavage enzyme, which carries out the first conversion in the steroidogenic pathway. In mammals, StAR controls this rate limiting step in steroidogenesis, and both StAR protein and StAR mRNA levels become rapidly elevated in response to tropic hormone stimulation. The relationship between StAR gene expression and steroid production in fish has not yet been well explored. We investigated the relationship between adrenocorticotropic hormone (ACTH)- and cAMP-stimulated cortisol production in vitro and levels of StAR transcripts in interrenal cells of rainbow trout. To assess the effect of ACTH on mRNA levels of a downstream steroidogenic enzyme, we also investigated the effects of ACTH on transcripts encoding 11beta hydroxylase (P450 11beta). In a series of experiments, juvenile rainbow trout head kidney tissue containing interrenal cells was incubated with either ACTH or dibutyryl cyclic AMP (dbcAMP). Cortisol in incubation media were measured by radioimmunoassay and total RNA was isolated from the tissue for Northern analysis or for quantitative real-time PCR...Continue Reading

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Citations

Nov 26, 2013·General and Comparative Endocrinology·J D JeffreyK M Gilmour
Feb 14, 2012·General and Comparative Endocrinology·Jennifer D JeffreyKathleen M Gilmour
Sep 18, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Dawoon Jung, Richard T Di Giulio
Sep 1, 2008·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Trond M KortnerAugustine Arukwe
Feb 3, 2009·Journal of Toxicology and Environmental Health. Part a·Trond M KortnerAugustine Arukwe
Jul 27, 2017·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Xiao-Hong LiuYao-Guang Zhang
Apr 18, 2013·Zebrafish·Anusha K S DhanasiriViswanath Kiron
Mar 23, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Amélie Gravel, Mathilakath M Vijayan
Dec 19, 2018·Frontiers in Endocrinology·Nataly SanhuezaSebastian Boltana
Jun 15, 2020·General and Comparative Endocrinology·Silvia Cristina CzuchlejNora Raquel Ceballos
Oct 19, 2012·The Journal of Experimental Biology·Marta Conde-SieiraJosé L Soengas

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