PMID: 43019Jan 1, 1979

Effects of actinomycin D on ribosomal RNA and protein synthesis as revealed by high resolution autoradiography

Virchows Archiv. B, Cell Pathology Including Molecular Pathology
A Martinez-Ramón

Abstract

Incorporation of tritiated amino acids and uridine was studied in untreated and actinomycin D treated HeLa cells by high resolution autoradiography. Results showed a non-selective inhibition of protein synthesis by actinomycin, as measured by the decrease in radioactive amino acid uptake. When cells pretreated with actinomycin D were incubated with radioactive amino acids and uridine, amino acid uptake in the nucleolus still occurred, while uridine uptake was almost completely eliminated. These findings suggest that in the absence of ribosomal RNA precursor synthesis, nucleolar protein synthesis continues to some extent, and that this protein is transported to the nucleolus.

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·L PuckettJ E Darnell
Nov 10, 1972·Nature·R H Singer, S Penman
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W Murphy, G Attardi
Oct 14, 1971·Journal of Molecular Biology·J C Maisel, E H McConkey
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R S WuJ R Warner
Oct 28, 1969·Journal of Molecular Biology·B E MadenJ E Darnell

Related Concepts

Cell Nucleolus
Ac-De
HeLa Cells
Ribosomes
15S RNA
Protein Biosynthesis

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