Effects of activated Bt transgene products (Cry1Ab, Cry3Bb) on immature stages of the ladybird Adalia bipunctata in laboratory ecotoxicity testing

Archives of Environmental Contamination and Toxicology
Jörg E U SchmidtAngelika Hilbeck

Abstract

Insect-active Bacillus thuringiensis (Bt) proteins are expressed by several transgenic crop plants to control certain pests, but nontarget organisms such as ladybirds also can be exposed to these proteins in the field. We developed an improved ecotoxicity testing protocol and conducted feeding trials in a laboratory setting to test for possible adverse effects of different concentrations of microbially produced trypsin-activated Cry1Ab and Cry3Bb toxins on the coccinellid Adalia bipunctata. Larval/pupal mortality, development time, and overall body mass accumulation were recorded. Even at the lowest concentration (5 microg/ml), A. bipunctata larvae fed with the lepidopteran-active Cry1Ab toxin exhibited significantly higher mortality than the control group. In experiments with the coleopteran-active Cry3Bb, only a concentration of 25 microg/ml resulted in a marginally significantly higher mortality compared to the control. Both experiments revealed a slight decline in mortality at the highest concentration of 50 microg/ml, though this was statistically significant only in the Cry1Ab treatment. No differences were detected for development time and body mass of newly emerged adults. Dilutions of the expression vector pBD10--used ...Continue Reading

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Citations

Jun 24, 2009·Transgenic Research·Agnès RicrochMarcel Kuntz
Jun 27, 2009·Environmental Science and Pollution Research International·Christoph Then
May 10, 2013·Nature Biotechnology·Jörg RomeisAnthony M Shelton
Apr 10, 2010·Science·Reyes Tirado, Paul Johnston
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Sep 14, 2017·Biotechnology & Genetic Engineering Reviews·Jonathan R LathamAngelika Hilbeck
Oct 25, 2013·Journal of Agricultural and Food Chemistry·Yan-Liang LiJing You

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