PMID: 7541691Apr 1, 1995Paper

Effects of acute and chronic ethanol on cyclic AMP accumulation in NG108-15 cells: differential dependence of changes on extracellular adenosine

British Journal of Pharmacology
E KellyR J Williams

Abstract

1. This study investigated the effects of acute and chronic ethanol on basal, agonist- and forskolin-stimulated cyclic AMP formation in NG108-15 mouse neuroblastoma x rat glioma hybrid cells, and examined the role of changes in extracellular adenosine concentrations on the effects observed. 2. NG108-15 cells incubated acutely with ethanol (1-200 mM) displayed concentration-dependent increases in basal and iloprost-stimulated (300 nM; a prostanoid IP receptor agonist) cyclic AMP accumulation but a concentration-dependent decrease in forskolin-stimulated (10 microM) accumulation. 3. Cells treated chronically with ethanol (200 mM) for 48 h displayed increases over control in basal, iloprost- (0.001-10 microM) and forskolin (0.01-100 microM)-stimulated cyclic AMP formation. However, chronic ethanol did not affect [3H]-iloprost binding to cell membranes. 4. Inclusion of adenosine deaminase (ADA; 1 unit ml-1) during the incubation period to measure cyclic AMP accumulation completely abolished the increase in basal accumulation following chronic ethanol, but did not affect the increase in iloprost stimulation. On the other hand ADA partially reversed the increase in forskolin stimulation following chronic ethanol, but even in the pres...Continue Reading

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Citations

Mar 19, 2004·Life Sciences·Annayya R Aroor, Shivendra D Shukla
May 26, 2005·Molecular and Cellular Neurosciences·Zhongqi LiCatherine Rivier
Feb 11, 2016·Journal of Oncology Practice·Hasan NadeemKiran K Turaga
Feb 12, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhongsheng PengBruce N Cronstein

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