PMID: 8967523May 1, 1996Paper

Effects of acute and chronic hypoxia on rat lung cyclooxygenase

The American Journal of Physiology
M Chida, N F Voelkel

Abstract

Cyclooxygenase-2 (COX-2) is an inducible cyclooxygenase enzyme and may play an important role in the pathogenesis of lung injury and in pulmonary vascular remodeling. In this study we determined the effects of acute or chronic hypoxia on COX-2 induction and its modulation by .NO and adenosine 3'-5'-cyclic monophosphate (cAMP). Isolated perfused rat lungs were exposed to a normoxic gas mixture or a hypoxic gas mixture for 3 h. Northern blot analysis showed that 3 h of acute hypoxia were sufficient to increase COX-2 but not COX-1 transcripts in the lung. COX-2 expression induced by acute hypoxia was enhanced by an inhibitor of nitric oxide synthase, N(G)-nitro-L-arginine methyl ester, and was suppressed by sodium nitroprusside, meclofenamate, and H-7 (an inhibitor of protein kinase A and C). COX-2 expression was also enhanced by dibutyryl cAMP and iloprost, a prostacyclin analogue. In contrast, 2 wk of chronic hypobaric hypoxia did not enhance COX-2 expression in the lung, but increased COX-2 protein levels, as assessed by Western blots. We conclude that acute hypoxia induces COX-2 gene expression in rat lung and that COX-2 induction by acute hypoxia is modulated by .NO, cAMP, and cyclooxygenase products. In particular, prostacyc...Continue Reading

Citations

Jun 14, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Lei XiRakesh C Kukreja
Mar 18, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alan E JonesJeffrey A Kline
Apr 28, 2011·The Journal of Pharmacology and Experimental Therapeutics·Jose G Lopez-LopezFrancisco Perez-Vizcaino
Oct 17, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Naveen K SomannaReza Izadpanah
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·C D FikeM Bousamra
Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·D ZhuE R Jacobs
Oct 22, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Candice D FikeSandra L Pfister
Sep 28, 2000·Journal of Applied Physiology·J L BerkR H Goldstein
Sep 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·D A BradburyA J Knox
Nov 2, 2004·Journal of Applied Physiology·Candice D FikeSandra L Pfister
Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·E R Jacobs, D C Zeldin
Apr 9, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·M HoriH Karaki

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