Effects of acute estradiol on 5-hydroxytryptamine and dopamine receptor subtype mRNA expression in female rat brain

Molecular and Cellular Neurosciences
B E H Sumner, G Fink

Abstract

The aim of the present study was to determine the effect of estradiol-17beta, in its positive feedback mode for the release of prolactin and luteinizing hormone (LH), on gene expression of 5-hydroxytryptamine (5-HT) and dopamine receptors. Gene expression was determined by measurement of the levels of receptor mRNA by in situ hybridization in brain sections from adult female rats. The animals were ovariectomized under halothane anesthesia on the morning of diestrus, given a sc injection of either estradiol benzoate (EB) or oil (vehicle), and killed between 1600-1700 h of the next day, presumptive proestrus. The injection of EB in this preparation is known to stimulate a massive surge of LH and prolactin in the late afternoon of presumptive proestrus. The injection of EB produced a significant increase in silver grain densities representing 5-HT(2) receptor mRNA in the dorsal raphe nucleus (DRN) and a significant reduction in these grain densities in the medial septum and diagonal band of Broca. The number of cells expressing 5-HT(2) receptor mRNA was increased by 290% in the DRN and decreased by 25% in the medial preoptic area. Estradiol had no effect on silver grain densities, or numbers or percentages of cells expressing 5-HT...Continue Reading

Citations

Oct 24, 1998·Clinical and Experimental Pharmacology & Physiology·G FinkR Rosie
Aug 21, 2015·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Mary V Seeman
Mar 19, 2014·Frontiers in Behavioral Neuroscience·Sean C Godar, Marco Bortolato
Nov 14, 1997·Neuroscience and Biobehavioral Reviews·L Uphouse
Oct 21, 2004·Brain Research·J GuptarakL Uphouse
Feb 6, 2017·Environmental Science and Pollution Research International·Chunhong LiuShaowen Huang
May 12, 2005·Behavioral and Cognitive Neuroscience Reviews·Zenab AminC Neill Epperson
Jul 1, 1996·The International Journal of Neuroscience·R Sandyk
Sep 26, 1996·Nature·G Fink, B E Sumner
Mar 5, 1999·Molecular Neurobiology·C L BetheaZ N Lu
Nov 13, 2009·Annals of the New York Academy of Sciences·Peter J Schmidt, David R Rubinow
Jun 1, 1996·Cellular and Molecular Neurobiology·G FinkJ P Quinn
Oct 6, 2005·Archives of Women's Mental Health·N Kurshan, C Neill Epperson
Feb 10, 2012·Der Nervenarzt·P BaldingerS Kasper
Oct 8, 1999·Psychosomatic Medicine·C N EppersonB Yamamoto
Jan 6, 2021·International Journal of Molecular Sciences·Wu Jeong HwangJun Soo Kwon
Feb 2, 2021·Frontiers in Neuroscience·Alanna DorseyKimberly J Jennings
Mar 22, 2002·Frontiers in Neuroendocrinology·Cynthia L BetheaJohn M Streicher
Sep 30, 2000·Brain Research. Brain Research Reviews·L Uphouse
Nov 10, 1998·Biological Psychiatry·D R RubinowC A Roca
Mar 21, 2007·Pharmacology, Biochemistry, and Behavior·Lynda UphouseJutatip Guptarak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.