Effects of acute glucose overload on histamine H2 receptor-mediated Ca2+ mobilization in bovine cerebral endothelial cells

Diabetes
C KimuraY Ito

Abstract

[Ca2+]i and whole-cell membrane current were measured in microvascular endothelial cells from bovine brain. The effects of histamine on [Ca2+]i were examined, and the acute effect of changing extracellular glucose concentration on Ca2+ homeostasis was investigated. Application of 10 micromol/l histamine evoked an initially transient and then sustained increase in [Ca2+]i in normal Krebs solution, but only the transient component in Ca2+-free solution, thereby indicating that histamine mobilizes Ca2+ both from intracellular store sites and extracellular space. The effects of histamine on [Ca2+]i were inhibited by the H2 antagonists, ranitidine and cimetidine, but not by the H1 antagonist, pyrilamine. Incubation of the cells for 2 h in solutions containing low (1.1 and 2.3 mmol/l) or high (23 mmol/l) concentrations of glucose did not influence the resting level of [Ca2+]i. Treatment with low concentrations of glucose did not impair histamine-induced Ca2+ mobilization. On the other hand, when histamine was applied to the cells pretreated with 23 mmol/l glucose, it failed to mobilize Ca2+ from both intracellular store sites and extracellular space. The effect of histamine was mimicked by dibutyryl cyclic AMP, but glucose overload f...Continue Reading

Citations

Aug 24, 2000·BJOG : an International Journal of Obstetrics and Gynaecology·B SzekacsE A Brinton
Dec 5, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Nikoleta DaskoulidouShang-Zhong Xu
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·C KimuraY Ito
Aug 3, 2019·International Journal of Molecular Sciences·Kenichi Goto, Takanari Kitazono
Dec 12, 2001·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·K C LeeC R Jan

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