PMID: 11312154Apr 20, 2001Paper

Effects of adenosine 5'-triphosphate and growth hormone on cellular H+ transport and calcium ion concentrations in cloned bovine mammary epithelial cells

The Journal of Endocrinology
K KatohY Obara

Abstract

The present experiment was carried out to investigate the effects of exogenous adenosine 5'-triphosphate (ATP) and growth hormone (GH) on cellular H(+) efflux rate (extracellular acidification rate) and Ca(2+) concentration ([Ca(2+)](c)) in cloned bovine mammary epithelial cells (bMEC) raised from the mammary gland of a 26-day-pregnant Holstein heifer. Perifusion of 2-day cultured cells with a medium containing ATP (10, 100 and 1000 micromol/l) for 30 min caused a significant and concentration-dependent increase in the cellular H(+) efflux rate. ATP application (100 micromol/l) caused a transient and large increase in [Ca(2+)](c) in all cells. In contrast, perifusion with a medium containing bovine GH at 10, 50 and 250 ng/ml for 30 min caused a significant decrease in the cellular H(+) efflux rate in a concentration-dependent manner. However, bovine GH application (50 ng/ml) caused a small decrease followed by an increase, in some cases, in [Ca(2+)](c). In bMEC treated with lactogenic hormones (1 microgram/l prolactin, 1 nmol/ml dexamethasone and 5 microgram/ml insulin) for 2 days, the increased H(+) efflux rate induced by ATP was significantly reduced, whereas the negative response induced by GH was inversely and significantly...Continue Reading

Citations

Jan 27, 2004·Biochemical and Biophysical Research Communications·T YonezawaY Obara
Jan 28, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Keitaro KitadeYoshiaki Obara
Nov 26, 2013·Purinergic Signalling·Geoffrey Burnstock
Jan 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Takahiro FujinoTokuo T Yamamoto
Oct 4, 2005·Domestic Animal Endocrinology·Shinichi YonekuraYoshiaki Obara
May 20, 2015·Autonomic Neuroscience : Basic & Clinical·George I Gorodeski
Aug 24, 2007·American Journal of Physiology. Cell Physiology·So Yeong LeeScott M O'Grady

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