Effects of adiponectin during in vitro maturation of goat oocytes: MEK 1/2 pathway and gene expression pattern

Reproduction in Domestic Animals = Zuchthygiene
Elizabete T GomesAndré M Batista

Abstract

Recent studies have shown that adiponectin, an adipokine predominantly produced by adipose tissue, regulates several reproductive processes. However, the mechanisms of action of adiponectin on the maturation of goat oocytes remain to be determined. The aim of this study was to investigate whether (a) adiponectin influences the meiotic maturation of goat oocytes; (b) MAPK MEK 1/2 mediates the effects of adiponectin; and 3) adiponectin differentially affects mRNA relative abundance of genes relevant for adiponectin signal transduction in goat oocytes. The addition of adiponectin (5 μg/ml) during the maturation of goat oocytes resulted in a higher percentage of successful nuclear maturation compared to those of the group without adiponectin (p < 0.05). Adiponectin-stimulated nuclear oocyte maturation was significantly impaired by a mitogen-activated protein kinase MEK 1/2 inhibitor, U0126 (p < 0.05). There was no evidence of any adiponectin-induced difference in the relative transcript abundances of AdipoR1, AdipoR2, AMPKα1, AMPKα2, PPARα and PPARγ genes. In conclusion, these results indicate that adiponectin has a positive effect on the meiotic maturation of goat oocytes through the MAPK MEK 1/2 pathway. Furthermore, the adiponec...Continue Reading

References

Feb 12, 2005·Journal of Animal Science·E LordM F Palin
Aug 19, 2006·Endocrinology·Sandra LedouxBruce D Murphy
May 17, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Christine ChabrolleJoélle Dupont
May 31, 2007·Molecular Endocrinology·Cheng-Guang LiangQing-Yuan Sun
Oct 10, 2007·International Journal of Obesity : Journal of the International Association for the Study of Obesity·D B CamposB D Murphy
Jul 22, 2008·Domestic Animal Endocrinology·Eugénie ChappazVilceu Bordignon
Jul 18, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Josef WanningerChrista Buechler
Mar 12, 2010·Reproductive Biology and Endocrinology : RB&E·Virginie MaillardJoëlle Dupont
Jul 1, 2010·Zygote : the Biology of Gametes and Early Embryos·Isana M A FrotaJosé R V Silva
Sep 29, 2012·Vitamins and Hormones·Marie-France PalinBruce D Murphy
Dec 5, 2012·Reproduction, Fertility, and Development·Yael Pomerantz, Nava Dekel
Feb 19, 2013·The International Journal of Developmental Biology·Joëlle DupontChristelle Ramé
Feb 23, 2013·Nature Reviews. Molecular Cell Biology·Rong Li, David F Albertini
Jan 15, 2014·Best Practice & Research. Clinical Endocrinology & Metabolism·Toshimasa YamauchiTakashi Kadowaki
Aug 4, 2015·Frontiers in Neuroscience·Michael J BertoldoPascal Froment
Feb 11, 2016·American Journal of Physiology. Endocrinology and Metabolism·Ágnes LendvaiRegina Ensenauer
Aug 26, 2016·PPAR Research·Maurizio VittiElisabetta Benedetti
Mar 24, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Agnieszka RakJoëlle Dupont

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Domestic Animal Endocrinology
Eugénie ChappazVilceu Bordignon
International Journal of Obesity : Journal of the International Association for the Study of Obesity
M NannipieriE Ferrannini
Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Mei WangYu An
© 2022 Meta ULC. All rights reserved