Effects of adipose-derived stem cells plus insulin on erectile function in streptozotocin-induced diabetic rats

International Urology and Nephrology
Feng ZhouJianquan Hou

Abstract

Erectile dysfunction (ED) is a distressing complication in men with diabetes mellitus (DM). This study aimed to investigate the effects of adipose-derived stem cells (ADSCs) plus insulin on ED in streptozotocin (STZ)-induced diabetic rats. Forty-five eight-week-old male Sprague-Dawley rats received intraperitoneal injection of STZ (60 mg/kg). Eight weeks after the induction, the determined diabetic rats were randomly distributed into four groups: rats in DM + PBS group received a one-time intracavernous (IC) injection of phosphate-buffered saline (PBS) solution, DM + ADSCs group received IC injection of ADSCs, DM + Insulin group received subcutaneous injection of neutral protamine Hagedorn twice a day, and DM + ADSCs + Insulin group received both ADSCs and neutral protamine Hagedorn treatments. Another 10 normal rats were served as control group and received IC injection of PBS. Four weeks after the treatments, intracavernous pressure, histopathological changes in penis, functional proteins of ADSCs, and penis were measured. We found that ADSCs expressed vascular endothelial growth factor, TIMP metallopeptidase inhibitor 1 (TIMP-1), and lipopolysaccharide-inducible CXC chemokine (LIX). ADSC injection partially restored cavernou...Continue Reading

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Citations

Oct 12, 2018·Expert Opinion on Biological Therapy·Serap GurWayne J G Hellstrom
Apr 19, 2017·International Urology and Nephrology·Quan-Liang HouWei-Dong Zhang
Jul 3, 2021·Journal of Clinical Medicine·Angelo CignarelliFrancesco Giorgino

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