PMID: 15348406Sep 7, 2004Paper

Effects of adsorbed heat labile serum proteins and fibrinogen on adhesion and apoptosis of monocytes/macrophages on biomaterials

Journal of Materials Science. Materials in Medicine
William G BrodbeckJames M Anderson

Abstract

A previously established human monocyte culture protocol was used to determine the effects of varying adsorbed proteins on monocyte/macrophage adhesion and survival on dimethyl-silane (DM) or RGD modified glass coverslips. Cells were allowed to adhere for 2 h in the absence of protein or in the presence of serum, fibrinogen (Fg), heat inactivated serum (HIS), serum supplemented with Fg or HIS with Fg. Cell adhesion and apoptosis rates were determined on days 0 (2 h), 3, 7 and 10 of culture. The presence of serum alone in the initial culture was sufficient to optimize monocyte/macrophage adhesion and survival rates. Adding Fg to serum did not increase adhesion nor decrease apoptotic rates. No protein or the addition of HIS during the initial incubation period significantly decreased monocyte/macrophage adhesion and survival on both surfaces, however, the addition of Fg to HIS restored adhesion and survival rates to those seen with in the presence of serum alone on RGD surfaces. These studies demonstrate that monocyte/macrophage adhesion and survival on biomaterial surfaces are optimized by adsorbed heat labile serum proteins while adsorbed Fg plays a surface property-dependent role.

Citations

Nov 13, 2007·Journal of Biomedical Materials Research. Part a·Amy K McNallyJames M Anderson
Jan 30, 2013·Macromolecular Bioscience·Sílvia GomesDavid L Kaplan
Feb 17, 2015·Journal of Biomedical Materials Research. Part a·Lisa M ChamberlainDavid W Grainger
Dec 1, 2014·Clinical and Translational Medicine·Hannah C BygdKaitlin M Bratlie
Jul 15, 2011·Journal of Materials Science. Materials in Medicine·Inês C GonçalvesBuddy D Ratner
Dec 3, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Yuji NaitoToshiharu Shinoka
Apr 2, 2011·Langenbeck's Archives of Surgery·Karsten JungePetra Lynen Jansen
Aug 6, 2005·Journal of Biomedical Materials Research. Part a·Jin-Ku KimDonald L Elbert
Jan 23, 2021·International Journal of Molecular Sciences·Andreea-Mariana NegrescuAnisoara Cimpean
Apr 4, 2021·Materials·Andreea-Mariana Negrescu, Anisoara Cimpean
Jul 25, 2006·Biophysical Journal·Gregor KnönerHalina Rubinsztein-Dunlop
Dec 15, 2020·ACS Applied Materials & Interfaces·Richard P TanSteven G Wise
Oct 6, 2021·Journal of Biomaterials Applications·Tijana Lužajić BožinovskiDanica Marković

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