PMID: 9541212Apr 16, 1998Paper

Effects of aFGF, bFGF, TGFbeta1 and IGF-I on odontoblast differentiation in vitro

European Journal of Oral Sciences
A MartínJ Aréchaga

Abstract

In this work, we investigated the effects of aFGF and bFGF alone or combined with TGFbeta1 or IGF-I on odontoblast differentiation. Trypsin-isolated dental papillae from day 17 mandibular first molar were cultured in semisolid-agar medium for 6 d. Our results demonstrated that aFGF, bFGF or combinations of these promoted cell polarization at the periphery of the dental papillae. Moreover, simultaneous addition of aFGF and TGFbeta1 to dental papillae cultures induced both polarization and functional differentiation of odontoblast-like cells, as well as extracellular matrix deposition. Combination of aFGF or bFGF with IGF-I caused cell polarization at the surface of dental papillae, but matrix secretion was restricted to a few explants. In the presence of bFGF and TGFbeta1, the explants had pronounced cell elongations but no matrix deposition. These results indicate that aFGF or bFGF is not able to induce odontoblast differentiation alone. However, both aFGF and bFGF can act synergistically with TGFbeta1 and IGF-I to strengthen their inductive effects and promote gradients of cytological and functional changes in odontoblast-like cells.

Citations

Feb 24, 2015·International Endodontic Journal·K H YeomT Nishihara
Apr 12, 2016·Frontiers in Cell and Developmental Biology·Maitane AurrekoetxeaFernando J Unda
Oct 21, 2009·Journal of Endodontics·Yoshio ShimabukuroShinya Murakami
Mar 2, 2016·Veterinary Pathology·P VogelR Brommage
Apr 21, 2005·Journal of Dental Research·D D Bosshardt
Feb 10, 2000·Journal of Dentistry·D TziafasH Lesot
Feb 22, 2005·Archives of Oral Biology·Javier CatónMargarita Zeichner-David
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A J Smith, H Lesot
Oct 18, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·G EmberyM Goldberg
Mar 19, 2003·Advances in Dental Research·I ThesleffJ Jernvall
Mar 19, 2003·Advances in Dental Research·H LesotJ V Ruch
Jul 6, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·F J UndaJ Aréchaga
Sep 5, 2002·Journal of Biomedical Materials Research·C LilliP Locci
Sep 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tamizchelvi Thyagarajan, Ashok B Kulkarni
Sep 7, 2007·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Zhimei Li, Varawan Sae-Lim
May 9, 2009·Journal of Anatomy·Javier Catón, Abigail S Tucker
Feb 8, 2014·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Xiaohua XieChunlin Qin
Sep 12, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·R BuchailleF Bleicher
Apr 27, 2010·Journal of Endodontics·Dimitrios Tziafas, Konstantinos Kodonas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.