Effects of Age and Estradiol on Gene Expression in the Rhesus Macaque Hypothalamus

Neuroendocrinology
Dominique H Eghlidi, H F Urbanski

Abstract

The hypothalamus plays a key role in mediating the effects of estrogen on many physiological functions, including reproduction, metabolism, and thermoregulation. We have previously observed marked estrogen-dependent gene expression changes within the hypothalamus of rhesus macaques during aging, especially in the KNDy neurons of the arcuate-median eminence (ARC-ME) that produce kisspeptin, neurokinin B, and dynorphin A. Little is known, however, about the mechanisms involved in mediating the feedback from estrogen onto these neurons. We used quantitative real-time PCR to profile age- and estrogen-dependent gene expression changes in the rhesus macaque hypothalamus. Our focus was on genes that encode steroid receptors (ESR1, ESR2, PGR, and AR) and on enzymes that contribute to the local synthesis of 17β-estradiol (E2; STS, HSD3B1/2, HSD17B5, and CYP19A). In addition, we used RT(2) Profiler™ PCR Arrays to profile a larger set of genes that are integral to hypothalamic function. KISS1, KISS1R, TAC3, and NPY2R mRNA levels increased in surgically menopausal (ovariectomized) old females relative to age-matched ovariectomized animals that received E2 hormone therapy. In contrast, PGR, HSD17B, GNRH2, SLC6A3, KISS1, TAC3, and NPY2R mRNA...Continue Reading

Citations

Dec 22, 2015·Neurobiology of Aging·Cynthia L BetheaHenryk F Urbanski
Jun 22, 2016·Molecular Neurobiology·Yu-Jie LaiGuo-Jun Chen
Dec 26, 2016·Peptides·Richard J Bodnar
Jun 16, 2017·Journal of Molecular Endocrinology·Dominique H EghlidiHenryk F Urbanski
May 11, 2018·Journal of Molecular Endocrinology·Dominique H EghlidiHenryk F Urbanski
Dec 23, 2017·Frontiers in Aging Neuroscience·Pauline GaignardRachida Guennoun

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