Effects of age and long term ovariectomy on prolactin secretion, as assessed by the reverse hemolytic plaque assay

Endocrinology
M A Sortino, P M Wise

Abstract

PRL secretion from pituitary lactotrophs was assessed using the reverse hemolytic plaque assay in young (2- to 3-month-old), middle-aged (10- to 12-month-old), and middle-aged long term ovariectomized (LT-OVX) rats to investigate whether 1) a change in the percentage of pituitary cells secreting PRL is detectable in middle-aged animals, 2) the amount of PRL secreted per cell changes with age, 3) aging involves a change in responsiveness to TRH and/or dopamine (DA), and 4) LT-OVX can prevent any of these changes. Young and middle-aged rats were OVX for 1 week. LT-OVX rats were OVX at 2-3 months of age and used when they were 10-12 months old. All animals were implanted with Silastic capsules containing estradiol (E2) in sesame oil and killed 3 or 8 days later. Anterior pituitaries were collected, and cells were dispersed and prepared for the reverse hemolytic plaque assay. Three days after E2 was implanted, the percentage of anterior pituitary cells that secrete PRL was higher in middle-aged compared to young rats. LT-OVX prevented this increase; the percentage of cells secreting PRL was significantly lower in LT-OVX than in both young and middle-aged rats. Basal secretion of PRL per cell was not different in young compared to m...Continue Reading

Citations

Mar 15, 1992·Mechanisms of Ageing and Development·J Fernandez-RuizJ A Ramos
Sep 1, 1991·Molecular and Cellular Endocrinology·P J Hornsby
Mar 1, 1991·Veterinary Pathology·R H Cardy
Jul 17, 2010·Journal of Clinical Periodontology·Daniel H FineMarcelo W B Araujo
Jan 17, 2004·International Dental Journal·M G Brading, P D Marsh
Jun 6, 2003·Journal of Clinical Periodontology·J-P Bernimoulin
Dec 11, 2002·Journal of Clinical Periodontology·Prem Sreenivasan, Abdul Gaffar

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