Effects of aging on gene expression and mitochondrial DNA in the equine oocyte and follicle cells

Reproduction, Fertility, and Development
Fernando Campos-ChillonElaine M Carnevale

Abstract

We hypothesised that advanced mare age is associated with follicle and oocyte gene alterations. The aims of the study were to examine quantitative and temporal differences in mRNA for LH receptor (LHR), amphiregulin (AREG) and epiregulin (EREG) in granulosa cells, phosphodiesterase (PDE) 4D in cumulus cells and PDE3A, G-protein-coupled receptor 3 (GPR3), growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15) and mitochondrial (mt) DNA in oocytes. Samples were collected from dominant follicles of Young (3-12 years) and Old (≥20 years) mares at 0, 6, 9 and 12h after administration of equine recombinant LH. LHR mRNA declined after 0h in Young mares, with no time effect in Old mares. For both ages, gene expression of AREG was elevated at 6 and 9h and EREG was expression was elevated at 9h, with higher expression in Old than Young mares. Cumulus cell PDE4D expression increased by 6h (Old) and 12h (Young). Oocyte GPR3 expression peaked at 9 and 12h in Young and Old mares, respectively. Expression of PDE3A increased at 6h, with the increase greater in oocytes from Old than Young mares at 6 and 9h. Mean GDF9 and BMP15 transcripts were higher in Young than Old, with a peak at 6h. Copy numbers of mtDNA did not vary...Continue Reading

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Citations

Apr 15, 2018·Equine Veterinary Journal·L H A Morris
Oct 3, 2019·Reproduction, Fertility, and Development·Lino Fernando Campos-ChillonJoy L Altermatt
Apr 27, 2017·Reproduction, Fertility, and Development·K A AlvesE L Gastal
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Achraf BenammarPascale Chavatte-Palmer

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Methods Mentioned

BETA
PCR

Software Mentioned

SAS
LightCycler 480
Oligoperfect
CLUSTALW

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