Abstract
Air discharge showed significant inhibition on mycelial growth and spore germination of Fusarium oxysporum, one of the main spoilage fungi in post-harvest lotus roots which is an important economic aquatic vegetable in China. However, the antimicrobial mechanism of air discharge is not clear yet. In the present study, the effects of air discharge on F. oxysporum separated from post-harvest rotten lotus roots were characterized by analyzing surface charges, cell wall permeability, and changes in chitin and chitosan including surface morphology, functional groups, degree of deacetylation, crystallinity, and C/N ratio. After air discharge treatments, alkaline phosphatase leak assay revealed that cell wall permeability of F. oxysporum was magnified. What's more, zeta potentials of F. oxysporum increased and negative charges on cell surfaces decreased. The ordered and compact molecular arrangements of chitin and chitosan in cell walls of F. oxysporum were reduced. The deacetylation degree of chitin and chitosan increased, and the C/N ratios of chitin and chitosan decreased. It was concluded from these results that air discharge caused the transformation in structures of chitin and chitosan, resulting in the exposure of positively ch...Continue Reading
References
Jun 1, 1997·Glycoconjugate Journal·R ShepherdA Falshaw
Feb 5, 1999·Journal of Molecular Biology·J SugiyamaT Watanabe
Jun 18, 2002·Journal of Applied Microbiology·L FanC F Forney
Aug 16, 2003·Gerontology·L O Simpson, D J O'Neill
May 6, 2004·Cancer Research·Eilon D KirsonYoram Palti
Aug 3, 2005·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Hans Merzendorfer
Sep 27, 2005·Critical Reviews in Food Science and Nutrition·Ajit K MahapatraJames L Julson
Nov 10, 2005·Eukaryotic Cell·Isaac R BanksJennifer K Lodge
Feb 1, 2006·The Journal of Antimicrobial Chemotherapy·Gerda M BruinsmaHenk J Busscher
Apr 19, 2007·BMC Microbiology·Louise A FletcherKevin G Kerr
Feb 4, 2010·Cancer Research·Neil M Goldenberg, Benjamin E Steinberg
Sep 21, 2010·International Journal of Food Microbiology·A K Tyagi, A Malik
Nov 18, 2010·The Journal of Antimicrobial Chemotherapy·Rachel L SoonJian Li
Feb 23, 2011·International Journal of Molecular Sciences·Kim-Chi HoangChun-Yi Liau
Mar 26, 2011·Journal of Cellular and Molecular Medicine·Yao-Xiong HuangMan Luo
Dec 1, 1999·Environmental Toxicology and Pharmacology·I DobrzyńskaZ Figaszewski
Oct 20, 2012·Critical Reviews in Biotechnology·Gurpreet Singh DhillonMausam Verma
Dec 6, 2014·Food Microbiology·Hae In YongCheorun Jo
Jan 18, 2016·Micron : the International Research and Review Journal for Microscopy·Rakkiyappan ChandranDennis LaJeunesse
Jul 13, 2016·ACS Applied Materials & Interfaces·Natthaporn KhamsenSiwapon Srisonphan
Jun 18, 2017·Folia Microbiologica·J JulákV Kříha
Dec 5, 2017·International Journal of Biological Macromolecules·Abdelghani HassainiaSami Boufi