Effects of ALA-PDT on biofilm structure, virulence factor secretion, and QS in Pseudomonas aeruginosa

Photodiagnosis and Photodynamic Therapy
Yang TanXia Lei

Abstract

To investigate the effects of ALA-PDT on biofilm structure, virulence factor secretion, and quorum sensing (QS) in Pseudomonas aeruginosa. We used confocal laser scanning microscopy (CLSM), an XTT assay, scanning electron microscopy (SEM), a virulence factor assay and qRT-PCR in this study. The XTT assay showed that ALA-PDT significantly inhibited the growth of planktonic P. aeruginosa. CLSM and SEM showed that ALA-PDT destroyed both bacterial and biofilm structures. The virulence factor assay showed that pyocyanin and elastase secretion were significantly inhibited in the ALA-PDT groups. qRT-PCR assays demonstrated that ALA-PDT significantly reduced the mRNA expression of QS-related genes. ALA-PDT kills planktonic and viable biofilm-associated P. aeruginosa cells, destroys biofilm structures, reduces virulence factor secretion and affects QS system gene expression.

Citations

Jul 17, 2019·AAPS PharmSciTech·Giovana Maria Fioramonti CalixtoCarla Raquel Fontana
Aug 5, 2020·Biomaterials Science·Xiaosong WeiXinge Zhang
Feb 29, 2020·Photochemistry and Photobiology·Rosane Bassi SoaresSilvia Cristina Nunez
Aug 28, 2020·Frontiers in Cellular and Infection Microbiology·Kaiyu ZhangYang Wang
Jun 13, 2020·Frontiers in Microbiology·Rita M PintoPatrick Van Dijck
Jun 18, 2020·Antibiotics·Adelaide AlmeidaMaria G P M S Neves
May 11, 2020·Biochemical Pharmacology·Gabriela Cervini BohmAdriana Casas

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