PMID: 16638297Apr 28, 2006Paper

Effects of all-trans retinoic acid on phenotype switch and migration of human pulmonary artery smooth muscle cells in vitro and the possible mechanisms

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases
Jun-yu LuShi-xin Chen

Abstract

To investigate the effects of all-trans retinoic acid (atRA) on phenotype switch and migration of human pulmonary artery smooth muscle cells (PASMC) in vitro and the possible mechanisms. Cultured human PASMC were divided into 8 groups randomly: group A (control group), group B (vehicle group), group C (treated with 10% fetal cattle serum), group D, group E, group F, group G, and group H (treated with atRA at the concentrations of 0.001 micromol/L, 0.01 micromol/L, 0.1 micromol/L, 1 micromol/L, 10 micromol/L respectively as well as 10% fetal cattle serum). Then, expression of JWA mRNA in human PASMC was detected by reverse transcriptase-polymerase chain reaction (RT-PCR), cell migration was measured using modified Boyden's chamber, and expression of SM-alpha-actin mRNA and protein was detected by RT-PCR and immunohistochemistry respectively. The difference between the groups was analyzed. There was no significant difference in the JWA mRNA expression of human PASMC between group A (0.125 +/- 0.014), group B (0.164 +/- 0.018) and group C (0.164 +/- 0.006), but the JWA mRNA expression in group D (0.326 +/- 0.018), group E (0.440 +/- 0.033), group F (0.460 +/- 0.040), group G (0.589 +/- 0.024) and group H (0.821 +/- 0.050) were sig...Continue Reading

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