PMID: 9550351Jan 1, 1996Paper

Effects of allopurinol on free-radical-induced reduction of the proliferation of retinal pigment epithelial cells

Documenta Ophthalmologica. Advances in Ophthalmology
A J AugustinS Hunt

Abstract

To investigate the effects of allopurinol on free-radical-induced reduction of growth of retinal pigment epithelial (RPE) cells. Bovine RPE cells were seeded at a density of 1 x 10(5) cells/Petri dish (60 x 15 mm) containing 4 ml MEM-Earle plus 15% FCS plus antibiotics. Twenty-four hours after seeding, cultures were exposed to a free radical generating system hypoxanthine(HX)/xanthine oxidase (XO) (HX: 0.1 micromol/l; XO: 700 microU/ml) for 10 or 60 min. Thereafter, the cells were washed. After washing the cells, allopurinol was added at 500 or 1000 micromol. To evaluate the effect of the radical generating system one group was not washed after treatment ('no wash'). The cultures were divided into eight groups: (1) no treatment (control); (2) HX/XO, no wash; (3,4) HX/XO, washed after 10 or 60 min; (5,6) HX/XO, washed after 10 or 60 min, application of 500 micromol of allopurinol; (7,8) HX/XO, washed after 10 or 60 min, application of 1000 micromol of allopurinol. Cell counts were carried out 96 h post-seeding. The values are expressed as means +/- SE. After 72 h (HX/XO, no wash), the radical generating system resulted in a significant decrease in cell growth as compared to controls. When being eliminated after 10 or 60 min, the...Continue Reading

References

Sep 1, 1991·Archives of Ophthalmology·M R LilesP D Oliver
Jan 1, 1987·Free Radical Research Communications·M GrootveldC P Moorhouse
May 29, 1986·Biochemical and Biophysical Research Communications·D A PetersonJ M Gerrard
Mar 9, 1987·FEBS Letters·P C MoorhouseJ M Gutteridge
Jan 1, 1996·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·A J AugustinM Spitznas

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