PMID: 11050691Oct 29, 2000Paper

Effects of allyl mercaptan and various allium-derived compounds on cholesterol synthesis and secretion in Hep-G2 cells

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
B H Cho, S Xu

Abstract

The present study was undertaken to compare the effects of allyl mercaptan (AM), a major metabolite of garlic, with several garlic constituents and extracts on cytotoxicity, cholesterol synthesis and its secretion in Hep-G2 cells. The cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), and treated with 5, 25, 50, 125, 250 and 500 microg/ml of AM, diallyl disulfide (DD), diallyl trisulfide (DT), steam-distilled garlic oil (SD) or vinyl-dithiin oil of garlic (VD) for 4 h. At concentrations up to 50 microg/ml, no significant cytotoxic effect was found in any group, but at concentrations above 250 microg/ml, the cell viability decreased drastically in all groups compared to the control. The treatment of cells with 25 microg/ml (non-cytotoxic concentration) of AM, DD, DT, SD for 4 h significantly inhibited [3H]acetate incorporation into cholesterol compared to that of the control (P < 0.05). The secretion of cholesterol into the medium was also significantly decreased in all groups except for VD. The treatment of cells with those allium constituents had no effect on either [3H]acetate incorporation into fatty acids or [3H]glycerol incorporation into triglyceride or phospholipid.

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Citations

Feb 9, 2011·Parasitology Research·Saleh Al-QuraishyMohamed Abdel Monam Dkhil
May 29, 2004·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Haim TapieroKenneth D Tew
Oct 22, 2003·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Kothapa N. ChettySushil K. Jain
May 31, 2002·Bioscience, Biotechnology, and Biochemistry·Gi-Nahm KimInshik Park
May 12, 2009·Chemico-biological Interactions·D TruongP J O'Brien
Nov 18, 2005·Journal of Dairy Science·M BusquetC Kamel
Jan 24, 2006·Journal of Dairy Science·M BusquetC Kamel
Nov 15, 2017·Future Science OA·Ravindra M SamarthYoshihisa Matsumoto
Nov 5, 2011·Biological Chemistry·Agnieszka BelterJan Barciszewski

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