Effects of Aloe vera and Eucalyptus methanolic extracts on experimental toxoplasmosis in vitro and in vivo

Experimental Parasitology
Bahareh MirzaalizadehMahbobeh Montazeri

Abstract

Toxoplasmosis is a worldwide disease caused by the protozoan parasite Toxoplasma gondii (T. gondii), which is most commonly treated by pyrimethamine and sulfadiazine. However, this treatment presents several adverse side effects; Thus, new drugs with lower toxicities are urgently needed. In this study the anti-T. gondii activity of A. vera and Eucalyptus extracts were evaluated in vitro using a MTT (3-(4, 5-dimethylthiazol-2-yl) 2, 5-diphenyltetrazolium bromide) assay and in vivo by measuring the survival rates of mice infected with 2 × 103 tachyzoites of RH strain of T. gondii and then injected intraperitoneally by different concentrations of extracts for 4 days. Biochemical parameters such as Ferric Reducing Antioxidant Potential (FRAP) and malondialdehyde (MDA) assay were also evaluated. As results, in the in vitro assay, the IC50 values were 13.2, 24.7, 2.63 μg/ml, and the selectivity indexes were 3.3, 2.4, 3.03 for the A. vera, Eucalyptus and pyrimethamine, respectively. The mice treated with Eucalyptus showed a better survival rate than others (P < 0.05). The increased weight of liver and spleen, due to infection, was reduced by treatments. In FRAP assay Eucalyptus showed a better antioxidant activity than the other extra...Continue Reading

Citations

Aug 14, 2019·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Maryam Fasihi KaramiVahid Raissi
Aug 14, 2019·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·L Khaleghi RostamkolaieM R Seifi Abadshapouri
Feb 15, 2020·Advanced Biomedical Research·Mahbobeh MontazeriSomayeh Shahani
Mar 17, 2020·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Daniel A Abugri, William H Witola
Mar 23, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Ghada Mohamed KadryDina M Hamdy El-Akkad
Jun 3, 2021·International Journal of Molecular Sciences·Karolina Szewczyk-GolecCelestyna Mila-Kierzenkowska
Jul 1, 2021·Parasitology·Letícia NishiAna Lúcia Falavigna-Guilherme
Oct 19, 2021·Frontiers in Cellular and Infection Microbiology·Na YaoSi-Yang Huang

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