Effects of alpha-(1,2)-fucosyltransferase genotype variants on plasma metabolome, immune responses and gastrointestinal bacterial enumeration of pigs pre- and post-weaning

PloS One
Ann-Sofie Riis PoulsenCharlotte Lauridsen

Abstract

In pigs, the alpha-(1,2) fucosyltransferase (FUT1) gene has been highlighted for its properties in controlling the intestinal expression of enterotoxigenic E. coli (ETEC) F18 receptors; a pathogen causing edema disease and post-weaning diarrhoea. In this study, we hypothesized that pigs with different genotypes (ETEC F18 resistant (FUT1AA) versus susceptible (FUT1AG)) differed in following systemic and enteric responses: growth performance, plasma metabolic profiles, expression of candidate genes for intestinal mucosal homeostasis and immunity, number of selected bacteria and the concentration of short-chain fatty acids (SCFA) in faeces and digesta in piglets pre and post-weaning, and on the ETEC F18 adherence ex vivo. Genotype had the strongest impact on plasma metabolomic profile on day 7 and 28 of age. FUT1AG piglets had higher level of N-methyl-2-pyrrolidinone, hippuric acid, oxindole, and 3-oxo-5-beta-chol-7-en-24-oic acid on day 7, and a higher level of guanosine on day 28 than that in the FUT1AA piglets. FUT1AA piglets had a higher level of betaine on day 7 and 3-methylguanine on day 28. On day 34 of age, the FUT1AA pigs had higher levels of S-2-hydroxyglutarate, L-phenylalanine, tauroursodeoxycholic acid and an undeterm...Continue Reading

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Citations

Sep 5, 2019·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·Francesca Romana MassacciChiara Francesca Magistrali
Feb 9, 2019·Journal of Animal Physiology and Animal Nutrition·Diana LuisePaolo Trevisi
May 23, 2020·Animals : an Open Access Journal From MDPI·Diana LuisePaolo Trevisi
Jan 18, 2019·Journal of Animal Science and Biotechnology·Robin B GuevarraHyeun Bum Kim
Jun 19, 2019·Journal of Animal Science and Biotechnology·Diana LuisePaolo Trevisi
May 28, 2019·Tropical Animal Health and Production·Rebeka SinhaBharat Bhushan
Feb 7, 2021·Microorganisms·Paolo TrevisiPaolo Bosi

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Methods Mentioned

BETA
genotyping
PCR
electrophoresis
PCA

Software Mentioned

Metabolomics
multcomp
R
Find Molecular Features
stats
pbkrtest
lme4
R R Development Core Team
DA
oTOF

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