PMID: 9422372Jan 9, 1998Paper

Effects of alpha-phenyl-tert-butylnitrone and selegiline on hydroxyl free radicals in rat striatum produced by local application of glutamate

Journal of Neurochemistry
B FergerK Kuschinsky

Abstract

The hydroxyl radical is a very reactive oxygen species that damages biomolecules in the brain and in other tissues. The possible pharmacological intervention to prevent hydroxyl radical formation was studied in vivo using the microdialysis technique in brains of nonanesthetized rats. Hydroxyl radicals form stable adducts [mainly 2,3-dihydroxybenzoic acid (2,3-DHBA) and 2,5-DHBA)] via an aromatic hydroxylation reaction with salicylic acid. 2,3-DHBA was separated and quantified by HPLC and electrochemical detection. Microdialysis probes were implanted into the striatum 1 day before measurement of levels of hydroxyl radicals. The next day, the probes were first perfused for 120 min with a modified Ringer's solution containing 5 mM salicylic acid, to obtain stable baselines. Afterward, the perfusion solution was switched to another solution that in addition contained 50 mM glutamate, to stimulate radical formation. Twenty minutes later, alpha-phenyl-tert-butylnitrone (PBN; 100 mg/kg), selegiline (10 mg/kg), or saline was administered intraperitoneally. The glutamate perfusion produced marked two- to 2.5-fold increases in 2,3-DHBA content. Treatment with PBN significantly antagonized the rise of 2,3-DHBA level, indicating that PBN i...Continue Reading

Citations

Mar 10, 2001·Antioxidants & Redox Signaling·Y Kotake
Sep 24, 1999·Journal of Neuroscience Research·I Cauquil-CaubèreG Barbanel
Aug 3, 2005·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Fang-Jung WanHui-Ching Lin
Feb 24, 2006·Neurochemistry International·Karuppagounder S SaravananKochupurackal P Mohanakumar

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