Effects of aluminum chloride on the kinetics of rat cortex synaptosomal ATP diphosphohydrolase (EC 3.6.1.5)

Biological Trace Element Research
M R SchetingerJ J Sarkis

Abstract

Aluminum chloride (AlCl3), a neurotoxic compound, inhibited ATP diphosphohydrolase activity of synaptosomes obtained from cerebral cortex of adult rats. The metal ion significantly inhibited ATPase and ADPase activities of the enzyme at all concentrations tested in vitro (0.01, 0.05, 0.5, 5, and 10 mM) in the presence of 1.5 mM calcium. When tested in the absence of Ca2+, and with increasing amounts of Al3+, enzyme activity remained below basal levels, suggesting that the trivalent cation Al3+ is not a substitute for the divalent cation Ca2+ in ATP-Ca2+ and ADP-Ca2+ complexes. The Al3+ inhibition was competitive with respect to Ca2+. The enzyme inhibition was reversed by the addition of deferoxamine (DFO). NaF significantly inhibited ATP diphosphohydrolase activity, and this inhibition was reversed by the addition of Ca2+ to the medium. Such inhibition was not potentiated by AlF4, which is an inhibitor of cation-transport ATPases.

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Citations

Sep 14, 2010·Cellular Immunology·Rosilene R KaizerJoão B T Rocha
Aug 10, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Rosilene Rodrigues KaizerMaria Rosa Chitolina Schetinger
Aug 7, 2007·Journal of Inorganic Biochemistry·Paula P Gonçalves, Virgília S Silva
Jul 21, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M R SchetingerS R Rodrigues
Nov 23, 2006·Molecular and Cellular Biochemistry·Vinícius Ribas MironMaria Rosa Chitolina Schetinger

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