Effects of amino acids and peptides on rumen microbial growth yields.

Journal of Dairy Science
J L Argyle, R L Baldwin

Abstract

Experiments were conducted using mixed rumen bacterial cultures to determine which amino acids limited growth. Complete amino acid mixtures stimulated microbial growth alone and when added to casein. Amino acid subgroups did not stimulate growth alone or when added to casein or casein hydrolysates. Results were similar whether growth was limited by periodic addition of low amounts of carbohydrate or when higher amounts were added to batch cultures. Little growth occurred with ammonia as sole N source. Addition of 100 mg/L of amino acids and peptides quadrupled growth, peptides at 10 mg/L resulted in higher growth than the corresponding amount of free amino acids. Apparent saturation of growth occurred when 10 mg/L of a complete amino acid mixture or trypticase was added to cultures. The Michaelis constant values for amino acids and trypticase were determined to be .5 and 1.0 mg/L, respectively. Growth was a linear function of amount of carbohydrate fermented with the coefficient of slope increasing with increasing amino acid concentrations. These experiments demonstrate that growth stimulation from amino acids and proteins is due to the number of amino acids provided in a given mixture rather than specific growth limiting amino...Continue Reading

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Citations

Jul 1, 2010·Animal : an International Journal of Animal Bioscience·S CalsamigliaA M van Vuuren
Oct 4, 2012·Animal : an International Journal of Animal Bioscience·A S SantosM A M Rodrigues
Apr 16, 2013·Applied and Environmental Microbiology·Timothy J HackmannJeffrey L Firkins
Oct 21, 2015·Animals : an Open Access Journal From MDPI·Albert Sundrum
Apr 1, 2013·Asian-Australasian Journal of Animal Sciences·Hui Ling ZhangYu Xia Yang
Jun 2, 2015·Frontiers in Microbiology·Timothy J Hackmann, Jeffrey L Firkins
May 10, 2017·FEMS Microbiology Ecology·César R V TeixeiraTimothy J Hackmann
Nov 21, 2017·Journal of Dairy Science·Mary Beth Hall, David R Mertens
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Jun 15, 2018·Journal of Animal Physiology and Animal Nutrition·Zora VáradyováKlaudia Čobanová
Dec 31, 2014·Applied and Environmental Microbiology·Bethany L DentonTimothy J Hackmann
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