Effects of an amyloid-beta 1-42 oligomers antibody screened from a phage display library in APP/PS1 transgenic mice

Brain Research
Jianping WangJian Wang

Abstract

We screened anti-Aβ1-42 antibodies from a human Alzheimer's disease (AD) specific single chain variable fragment (scFv) phage display library and assessed their effects in APP/PS1 transgenic mice. Reverse transcription-PCR was used to construct the scFv phage display library, and screening identified 11A5 as an anti-Aβ1-42 antibody. We mixed 11A5 and the monoclonal antibody 6E10 with Aβ1-42 and administered the mixture to Sprague-Dawley rats via intracerebroventricular injection. After 30 days, rats injected with the antibody/Aβ1-42 mixture and those injected with Aβ1-42 alone were tested on the Morris water maze. We also injected 11A5 and 6E10 into APP/PS1 transgenic mice and assessed the concentrations of Aβ in brain and peripheral blood by ELISA at 1-month intervals for 3 months. Finally we evaluated behavior changes in the Morris water maze. Rats injected with Aβ1-42 and mixed antibodies showed better performance in the Morris water maze than did rats injected with Aβ1-42 alone. In APP/PS1 transgenic mice, Aβ concentration was lower in the brains of the antibody-treated group than in the control group, but higher in the peripheral blood. The antibody-treated mice also exhibited improved behavioral performance in the Morris ...Continue Reading

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Citations

Apr 14, 2019·British Journal of Pharmacology·Erika CeconRalf Jockers
Jul 4, 2017·Journal of Neurochemistry·Adriano SebollelaWilliam L Klein
Mar 5, 2019·Journal of Biological Engineering·Briana I Martinez, Sarah E Stabenfeldt
Dec 30, 2017·Experimental and Therapeutic Medicine·Lu LiuGuo-Jun Chen
Jun 2, 2021·Applied Microbiology and Biotechnology·Zheng ZhangFei Xiao

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