Effects of an intrahypothalamic injection of antisense oligonucleotides for preproenkephalin mRNA in female rats: evidence for opioid involvement in lordosis reflex

Brain Research
A NicotD W Pfaff

Abstract

Previous studies in female rats have shown that estrogen increases preproenkephalin (PPE) mRNA levels in the ventrolateral part of the ventromedial nucleus of the hypothalamus (VMHVL), an area implicated in the modulation of sexual behavior. In order to assess the physiological role of hypothalamic opioid expression in lordosis reflex 16-mer oligodeoxynucleotide (ODN) directed towards the PPE mRNA were acutely microinjected above the VMH of estradiol-primed ovariectomized rats. Estradiol-induced lordosis behavior was observed in response to a stud male 2 days thereafter. Antisense (without or with 4 mismatches) ODN injections near the VMHVL resulted in a significant reduction in lordosis quotient compared to control (reverse sense) ODN treatment or to antisense ODN injections targeted anterior or posterior to the VMHVL. In contrast, locomotor activity of these animals in the open-field test was not affected by ODN treatments. Enkephalin immunoreactive levels were determined by radioimmunoassay in the preoptic area, a major terminal field of the VMHVL. Estradiol-induced enkephalin levels were greatly reduced in antisense-treated groups. Using the in situ hybridization technique, PPE mRNA levels in the VMHVL were also determined....Continue Reading

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Citations

Mar 8, 2006·Reviews in the Neurosciences·Thierry D Charlier, Jacques Balthazart
Apr 7, 1999·Antisense & Nucleic Acid Drug Development·A W Szklarczyk, L Kaczmarek
Mar 21, 2002·Journal of Cardiovascular Pharmacology·Li Lin, Wen-Jun Yuan
Mar 21, 2006·Journal of Neuroscience Research·Tadashi KitaharaTakeshi Kubo
Jan 22, 2000·Neuroscience and Biobehavioral Reviews·A Argiolas
Jun 14, 2006·Physiology & Behavior·Nino DevidzeDonald W Pfaff
Feb 23, 2011·Frontiers in Neuroendocrinology·Loretta M Flanagan-Cato

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