Effects of an opal termination codon preceding the nsP4 gene sequence in the O'Nyong-Nyong virus genome on Anopheles gambiae infectivity.

Journal of Virology
Kevin M MylesAnn M Powers

Abstract

The genomic RNA of an alphavirus encodes four different nonstructural proteins, nsP1, nsP2, nsP3, and nsP4. The polyprotein P123 is produced when translation terminates at an opal termination codon between nsP3 and nsP4. The polyprotein P1234 is produced when translational readthrough occurs or when the opal termination codon has been replaced by a sense codon in the alphavirus genome. Evolutionary pressures appear to have maintained genomic sequences encoding both a stop codon (opal) and an open reading frame (arginine) as a general feature of the O'nyong-nyong virus (ONNV) genome, indicating that both are required at some point. Alternate replication of ONNVs in both vertebrate and invertebrate hosts may determine predominance of a particular codon at this locus in the viral quasispecies. However, no systematic study has previously tested this hypothesis in whole animals. We report here the results of the first study to investigate in a natural mosquito host the functional significance of the opal stop codon in an alphavirus genome. We used a full-length cDNA clone of ONNV to construct a series of mutants in which the arginine between nsP3 and nsP4 was replaced with an opal, ochre, or amber stop codon. The presence of an opal...Continue Reading

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Citations

Dec 3, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kevin M MylesZach N Adelman
Sep 2, 2008·Vector Borne and Zoonotic Diseases·Christopher N MoresCindi A Rossi
Dec 23, 2011·Journal of Virology·N L ForresterW I Lipkin
Jun 19, 2015·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Tit-Yee Wong, Steve D Schwartzbach
Mar 15, 2015·Nucleic Acids Research·Mary Etna HaacZach N Adelman
Oct 10, 2014·PLoS Neglected Tropical Diseases·Jeremy P LedermannAnn M Powers
Jun 21, 2014·Cell·Sandeepa M EswarappaPaul L Fox
Aug 23, 2017·Pathogens and Global Health·Giovanni RezzaScott C Weaver
Sep 23, 2018·The Journal of Biological Chemistry·Joseph A KendraJonathan D Dinman
Jul 17, 2020·Frontiers in Microbiology·Marcela S CunhaLuciana J da Costa
Jul 3, 2021·Pathogens·Autumn T LaPointe, Kevin J Sokoloski
Jul 29, 2021·Journal of Virology·Laura Sandra LelloAge Utt

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