Effects of angiotensin II and insulin on ERK1/2 activation in fibroblasts from hypertensive patients

American Journal of Hypertension
Michelangelo SartoriAndrea Semplicini

Abstract

Insulin resistance, a frequent finding in hypertensive patients, leads to accelerated cardiovascular damage. It has been suggested that a crosstalk between angiotensin II and insulin signaling pathways may provoke insulin resistance, and may contribute to the development of cardiovascular damage. To identify a common pathophysiologic pathway between metabolic disorders and cardiovascular remodeling, we investigated the effect of angiotensin II and insulin on extracellular signal regulated kinases 1 and 2 (ERK1/2), isoforms of mitogen-activated protein kinases (MAPK) involved in cellular proliferation and extracellular matrix deposition. Skin fibroblasts from normotensive subjects, insulin sensitive hypertensive subjects, and insulin resistant hypertensive subjects were cultured and used after four passages. The ERK1/2 expression and phosphorylation were measured by Western blot using specific antibodies, respectively anti-ERK1/2 and anti-pERK1/2. Expression of AT1 receptor for angiotensin II was determined by reverse transcriptase-polymerase chain reaction in real time. The ERK1/2 were similarly expressed in skin fibroblasts from all groups; ERK1/2 phosporylation evoked by angiotensin II was significantly higher in fibroblasts ...Continue Reading

Citations

May 24, 2006·Heart·E IngelssonJ Sundström
Sep 14, 2011·Diabetes Care·Angela SciacquaFrancesco Perticone
Sep 13, 2008·Cardiovascular Therapeutics·Aaron K F WongChim C Lang
Feb 14, 2014·Journal of Cardiovascular Medicine·Giuseppina NovoSalvatore Novo
Oct 29, 2009·Orvosi hetilap·András Nagy, Zsuzsanna Cserép
Mar 15, 2006·Journal of Hypertension·Michelangelo SartoriAndrea Semplicini
May 6, 2019·Endocrine Reviews·Antonino Di Pino, Ralph A DeFronzo
Sep 23, 2016·Archives of Dermatological Research·T EmanuelliE Carvalho
Jul 6, 2021·Frontiers in Cardiovascular Medicine·Shaomin YangHaixiong Chen

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