Effects of antibodies against cell surface protein antigen PAc-glucosyltransferase fusion proteins on glucan synthesis and cell adhesion of Streptococcus mutans.

Infection and Immunity
H YuT Koga

Abstract

Cell surface protein antigen (PAc) and glucosyltransferases (GTFs) produced by Streptococcus mutans are considered to be major colonization factors of the organism, and the inhibition of these two factors is predicted to provide protection against dental caries. In this study, we have constructed fusion protein PAcA-GB, a fusion of the saliva-binding alanine-rich region (PAcA) of PAc with the glucan binding (GB) domain of GTF-I, an enzyme catalyzing the synthesis of water-insoluble glucan from sucrose, and fusion protein PAcA-SB, a fusion of PAcA with the sucrose binding (SB) domain of GTF-I. The recombinant fusion proteins were purified from cell extracts of Escherichia coli harboring the fusion genes, and rabbit antibodies against these fusion proteins were prepared. Water-insoluble glucan synthesis by cell-associated and cell-free GTF preparations from S. mutans as well as total glucan synthesis by GTF-I was markedly inhibited by anti-PAcA-GB immunoglobulin G (IgG) antibodies but not by anti-PAcA-SB IgG antibodies. Significant inhibition of the sucrose-independent and sucrose-dependent adhesion of S. mutans to saliva-coated hydroxyapatite beads was observed when anti-PAcA-GB antibodies were added to the reaction mixture. Ant...Continue Reading

References

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Citations

Nov 1, 2001·Clinical and Diagnostic Laboratory Immunology·Y ShimazakiT Koga
Apr 23, 2005·Infection and Immunity·Daniel J SmithMartin A Taubman
May 27, 2006·Nature Reviews. Immunology·Martin A Taubman, David A Nash
Aug 22, 2003·Oral Microbiology and Immunology·F LiN K Childers
Jul 21, 2007·Acta Pharmacologica Sinica·Feng ZhangQi-wei Tian
Jan 12, 2007·FEMS Microbiology Letters·David J LynchJeffrey A Banas
Apr 16, 1999·Oral Microbiology and Immunology·G Hajishengallis, S M Michalek
Mar 13, 2019·Molecular Oral Microbiology·Takahiko Oho, Emi Nagata
Aug 23, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D J Smith
May 24, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J A Banas, M M Vickerman
Sep 14, 2019·Letters in Applied Microbiology·M Patel
Apr 26, 2002·Vaccine·Toshihiko KogaYoshio Nakano

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