Effects of Antioxidants in Reducing Accumulation of Fat in Hepatocyte

International Journal of Molecular Sciences
Jung-Pyo YangEun-Hee Shin

Abstract

The progress of the hepatic steatosis (HS), a clinicopathological status, is influenced by cellular oxidative stress, lipogenesis, fatty acid (FA) oxidation, and inflammatory responses. Because antioxidants are gaining attention as potent preventive agents for HS, we aimed to investigate anti-lipogenic effects of the antioxidants vitamin C (VC), N-acetylcysteine (NAC), and astaxanthin (ATX) using hepatocytes. For this, we established an in vitro model using 1 mM oleic acid (OA) and human liver hepatocellular carcinoma (HepG2) cells; 10 μM antioxidants were evaluated for their ability to reduce fat accumulation in hepatocytes. Our results showed that all three antioxidants were effective to reduce fat accumulation for the molecular targets such as reduction in lipid droplets, triglyceride (TG) concentration, reactive oxygen species (ROS) production, and cell apoptosis, as well as in gene expressions of endoplasmic reticulum (ER) stress-related effectors, lipogenesis, and inflammatory cytokines. There were simultaneous increases in diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect, cell survival, AMPK phosphorylation, NRF2-related gene expression for cellular defense, and FA β-oxidation. However, among these, ATX more ef...Continue Reading

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Citations

Jul 15, 2020·Marine Drugs·Rebecca LandonFani Anagnostou
Aug 6, 2020·Phytotherapy Research : PTR·Sajad FakhriMohammad Hosein Farzaei
Sep 25, 2019·Marine Drugs·Suhn Hyung Kim, Hyeyoung Kim
Aug 11, 2020·International Journal of Food Science·Tatas Hardo Panintingjati Brotosudarmo Heriyanto
Oct 3, 2020·Antioxidants·Nimmy VargheseAnne Eckert
Jul 2, 2020·Drug Design, Development and Therapy·Jingjing LiJianye Wu
Aug 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mei-Chih TsaiChin-Lin Hsu
Sep 29, 2021·Critical Reviews in Food Science and Nutrition·Yunrui CaoJie Xu

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Methods Mentioned

BETA
PCR
fluorescence-activated cell sorting
FACS
electrophoresis
Assay

Software Mentioned

ImageJ
Excel
GraphPad Prism
FlowJo
GraphPad

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