Abstract
This study aimed to investigate effects of apple polyphenols (APPs) on myofiber-type transformation in longissimus dorsi muscle of finishing pigs and its mechanism. In this study, 36 healthy castrated Duroc × Landrace × Yorkshire pigs with an average body weight of 71.25 ± 2.40 kg were randomly divided into three treatment groups (control, 0.04% APPs, 0.08% APPs). The experiment lasted for 49 days. Results showed that dietary APP supplementation increased the protein expression of MyHC I and the activities of succinic dehydrogenase and malate dehydrogenase, as well as decreased the protein expression of MyHC IIb and the activity of lactate dehydrogenase, suggesting that APPs promoted muscle fiber-type transformation from fast-twitch to slow-twitch in finishing pigs. We also showed that dietary 0.08% APP supplementation increased the expressions of mitochondrial biogenesis and function-related proteins PGC-1α, Sirt1 and Cytc. In addition, dietary supplementation with 0.08% APPs increased the activities of T-SOD, GSH-PX and CAT and decreased the MDA content. Together, we provided the first evidence that APP promotes muscle fiber-type transformation from fast-twitch to slow-twitch in finishing pigs, which may be achieved by improv...Continue Reading
References
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 16, 2002·Nature·Jiandie LinBruce M Spiegelman
Sep 11, 2007·Carcinogenesis·Tomisato MiuraTatsusuke Sato
Apr 21, 2009·Cell Biology International·Min Lu, Xingguo Gong
Jun 30, 2009·Biochimica Et Biophysica Acta·Jin-Qiang ChenJames D Yager
Aug 19, 2010·Planta medica·Tadahiro SunagawaTakuji Shirasawa
May 5, 2011·Journal of Inherited Metabolic Disease·Adriana P RebeloCarlos T Moraes
Feb 4, 2012·Environmental Toxicology and Pharmacology·Alzbeta StaraJosef Velisek
May 9, 2012·Cell Metabolism·Nathan L PriceDavid A Sinclair
Jun 29, 2015·Free Radical Biology & Medicine·Takafumi Suzuki, Masayuki Yamamoto
Jul 30, 2015·PloS One·Wataru MizunoyaRyuichi Tatsumi
Dec 8, 2015·Journal of Animal Science·A F JiaQ Q Peng
Jul 16, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Wataru MizunoyaRyuichi Tatsumi
Oct 27, 2017·Animal : an International Journal of Animal Bioscience·T FangG Liu
May 10, 2018·Scientific Reports·Isao MasudaTakahiko Shimizu
May 17, 2019·Food & Function·Qinyang JiangYanna Huang
Jun 30, 2019·Animal Science Journal = Nihon Chikusan Gakkaihō·Xiaoling ChenZhiqing Huang
Oct 30, 2019·Food & Function·Xiaojiao XuZhiqing Huang
Feb 2, 2020·The Journal of Nutritional Biochemistry·Wanxue WenBing Yu