PMID: 8950029Nov 1, 1996Paper

Effects of Ara-C on neutral sphingomyelinase and mitogen- and stress-activated protein kinases in T-lymphocyte cell lines

Biochemistry and Molecular Biology International
C D BradshawK E Meier

Abstract

Neutral sphingomyelinase (SMase) can be activated by extracellular signals to produce ceramide, which may affect mitogen-activated protein kinase (MAPK) activities. Neutral SMase activity was assessed in membranes from Jurkat, a human T-cell line, and EL4, a murine T-cell line. Ara-C activated SMase with 10 minutes in both Jurkat and EL4 cells, while phorbol ester (PMA) had no effect. PMA, but not Ara-C or ceramides, activated ERK MAPKS, in Jurkat and EL4. PMA acted synergistically with ionomycin to activate JNK MAPKs in Jurkat and EL4 within 10 minutes. Ara-C activated JNKs only after prolonged incubation (90-120 minutes). Thus, ceramide is not a positive signal for ERK activation in T-cell lines. The effects of Ara-C on JNK activity may be mediated through secondary response pathways.

Citations

Jun 13, 1998·Progress in Lipid Research·L RiboniG Tettamanti
Oct 19, 2002·Biochimica Et Biophysica Acta·Anne-Dominique TerrisseJean-Pierre Jaffrézou
Apr 27, 1999·Biochimica Et Biophysica Acta·T Levade, J P Jaffrézou
Jan 5, 2014·Biochimica Et Biophysica Acta·Jean-Philip TrumanYusuf A Hannun
Feb 24, 2015·Apoptosis : an International Journal on Programmed Cell Death·Sehamuddin GaladariFaisal Thayyullathil
Sep 19, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Solène GrazideJean-Pierre Jaffrézou
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C BezombesJ P Jaffrézou

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