Effects of arabinosylcytosine-substituted DNA on DNA/RNA hybrid stability and transcription by T7 RNA polymerase

Biochemistry
T Mikita, G P Beardsley

Abstract

Cytosine arabinoside (araC) is a potent antileukemic agent which interferes with DNA replication both as a dNTP competitive inhibitor as well as after its misincorporation into DNA. We previously developed a chemical methodology for the synthesis of DNA oligomers containing araC which allowed us to study its site specific effects on duplex stability and chemical reactivity [Beardsley, G. P., Mikita, T., Klaus, M., & Nussbaum, A. (1988) Nucleic Acids Res. 16, 9165], as well as its effects on DNA ligase and DNA polymerase activity [Mikita, T., & Beardsley, G. P. (1988) Biochemistry 27, 4698]. The DNA polymerase studies, in addition to other observations, showed that araC in DNA templates could have an inhibitory effect on polymerase bypass. As a template lesion, there exists the potential for interference with other aspects of DNA metabolism, such as transcription. We have characterized a DNA/RNA hybrid containing an araC-G base pair, comparing thermal stability, chemical cleavage rates, and duplex gel mobility to an identically sequenced DNA duplex. We find that the A-form DNA/RNA hybrid and the B-form DNA duplex are nearly identical in the extent their thermal stability is affected by an araC-G(dG) base pair. Substitutions of a...Continue Reading

References

Jan 1, 1977·Annual Review of Biochemistry·N R Cozzarelli
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M EgliA Rich
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Kunala, D E Brash
Nov 20, 1992·Journal of Molecular Biology·L GrossW T McAllister
Mar 20, 1992·Journal of Molecular Biology·R SousaE M Lafer
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C P Selby, A Sancar
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y H ChenD F Bogenhagen
Jan 1, 1990·Pharmacology & Therapeutics·G E Wright, N C Brown
Feb 1, 1990·Nature·A BhattacharyyaD M Lilley
Dec 22, 1986·Nucleic Acids Research·H T SteelyR L Ratliff
Jun 28, 1988·Biochemistry·T Mikita, G P Beardsley
Oct 11, 1988·Nucleic Acids Research·G P BeardsleyA L Nussbaum
Jan 1, 1987·Annual Review of Biochemistry·P Modrich
May 31, 1988·Biochemistry·C T MartinJ E Coleman
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R A Ikeda, C C Richardson
Nov 11, 1987·Nucleic Acids Research·J F MilliganO C Uhlenbeck
Jan 1, 1984·Annual Review of Biochemistry·P H von HippelJ A McSwiggen
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W Zhou, P W Doetsch
Aug 20, 1993·Journal of Molecular Biology·L E MacdonaldW T McAllister
Oct 5, 1993·Journal of Molecular Biology· Fedoroff OYuB R Reid
Feb 20, 1993·Journal of Molecular Biology·G A DiazW T McAllister

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Citations

Jun 2, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christopher K Mathews
Nov 9, 2015·International Journal of Cancer. Journal International Du Cancer·Anna WojtuszkiewiczJacqueline Cloos
Jan 15, 2004·Molecular Pharmacology·William R Hartman, Patricia Hentosh
Mar 21, 2003·The Journal of Biological Chemistry·Virginia S KalogerakiPhilip C Hanawalt
Dec 8, 2004·Archives of Pathology & Laboratory Medicine·Ximbo Zhang, Frederick L Kiechle

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