PMID: 19939215Nov 27, 2009Paper

Effects of arginine on photostability and thermal stability of IgG1 monoclonal antibodies

Current Pharmaceutical Biotechnology
Haripada MaityJoel Goldstein

Abstract

This study demonstrates that arginine is a highly effective solvent additive which significantly reduces the light induced aggregation of four IgG1 type monoclonal antibodies (named as IMC-1A, IMC-1B, IMC-1C and IMC-1D) as measured by size exclusion chromatography. All experiments were performed in a phosphate buffer system containing either sodium chloride or arginine hydrochloride. The protein samples were exposed to light in a photo chamber according to ICH (International Conference on Harmonization) guidelines. Thermal unfolding transition temperature (T(m)) of IMC-1A as determined by differential scanning calorimetry (DSC) was significantly decreased ( approximately 3.3 degrees C) in the presence of arginine hydrochloride as compared to in sodium chloride. However, a noticeable increase in thermal stability was observed for IMC-1B, IMC-1C and IMC-1D in the presence of arginine hydrochloride. The photostability of all these molecules was significantly enhanced by arginine hydrochloride and both a direct and inverse correlation was observed between conformational stability and photostability. To our knowledge, this paper for the first time, demonstrates that arginine hydrochloride considerably reduces the light induced aggre...Continue Reading

Citations

Jul 26, 2012·The Journal of Physical Chemistry. B·Wayne G LilyestromThomas M Scherer
Dec 29, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Alavattam SreedharaRavuri S K Kishore
Oct 2, 2012·Archives of Biochemistry and Biophysics·Christopher M Johnson
Oct 13, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Patrick GaridelSven Bahrenburg
Feb 22, 2018·Pharmaceutical Research·Ming LeiChristian Schöneich
Feb 19, 2021·Critical Reviews in Biotechnology·Harleen Kaur
Aug 30, 2019·Journal of Pharmaceutical Sciences·Yoann Le BasleValérie Sautou
Apr 24, 2012·Journal of the American Chemical Society·Rock J ManciniHeather D Maynard
Jun 2, 2020·Journal of Chemical Information and Modeling·Santanu Santra, Madhurima Jana
Jun 20, 2013·Biomacromolecules·Juneyoung LeeHeather D Maynard
Feb 4, 2012·Molecular Pharmaceutics·Bruce D MasonBruce A Kerwin
Jan 13, 2017·Journal of the American Chemical Society·Emma M Pelegri-O'DayHeather D Maynard

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