Effects of Arginine on Streptococcus mutans Growth, Virulence Gene Expression, and Stress Tolerance

Applied and Environmental Microbiology
Brinta Chakraborty, Robert A Burne

Abstract

Streptococcus mutans is a common constituent of oral biofilms and a primary etiologic agent of human dental caries. The bacteria associated with dental caries have potent abilities to produce organic acids from dietary carbohydrates and to grow and metabolize in acidic conditions. By contrast, many commensal bacteria produce ammonia through the arginine deiminase system (ADS), which moderates the pH of oral biofilms. Arginine metabolism by the ADS is a significant deterrent to the initiation and progression of dental caries. In this study, we observed how exogenously provided l-arginine affects the growth, the virulence properties, and the tolerance of environmental stresses of S. mutans Supplementation with 1.5% arginine (final concentration) had an inhibitory effect on the growth of S. mutans in complex and chemically defined media, particularly when cells were exposed to acid or oxidative stress. The genes encoding virulence factors required for attachment/accumulation (gtfB and spaP), bacteriocins (nlmA, nlmB, nlmD, and cipB), and the sigma factor required for competence development (comX) were downregulated during growth with 1.5% arginine. Deep sequencing of RNA (RNA-Seq) comparing the transcriptomes of S. mutans growing ...Continue Reading

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Citations

Jan 23, 2018·Advances in Dental Research·R A Burne
Mar 20, 2020·Molecular Microbiology·Tridib GangulyJosé A Lemos
Jan 29, 2019·Frontiers in Microbiology·Jonathon L Baker, Anna Edlund
Oct 15, 2019·Frontiers in Microbiology·Zhiyan HeWei Zhou
Jan 29, 2019·BioMed Research International·Xinyi KuangXin Xu
May 16, 2020·Scientific Reports·Mohammed Nadeem BijleCynthia Kar Yung Yiu
Dec 20, 2020·Polymers·Mohammed Nadeem BijleCynthia Yiu
Apr 17, 2021·BioMed Research International·Yan SunKeke Zhang
Apr 5, 2019·Caries Research·Egija Zaura, Svante Twetman

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